Quantification of complement C5b-9 binding to cells by flow cytometry
Moskovich, O; Fishelson, Z
| HERO ID | 2900085 |
|---|---|
| In Press | No |
| Year | 2014 |
| Title | Quantification of complement C5b-9 binding to cells by flow cytometry |
| Authors | Moskovich, O; Fishelson, Z |
| Journal | Methods in Molecular Biology |
| Volume | 1100 |
| Page Numbers | 103-108 |
| Abstract | Interaction of the complement system, directly or indirectly (e.g., via antibodies), with cells activates the early and late complement components and culminates in the deposition of a membrane-spanning C5b-9 complex on the cell surface. At a high copy number, this C5b-9 will activate cell death, whereas at a low copy number, it will transmit various signals into cells. Quantification of C5b-9 deposition is useful for assessments of the capacity of cells and antibodies to activate complement. By using an antibody that identifies a novel antigen of the C5b-9 complex, the amount of C5b-9 complexes on cells can be quantified by flow cytometry. The detailed protocol is described in this chapter. |
| Doi | 10.1007/978-1-62703-724-2_8 |
| Pmid | 24218253 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |