Spectrophotometric Determination of Circulating Cholinesterases in Rats
Matthew, CB; Chapin, CL
| HERO ID | 2777889 |
|---|---|
| In Press | No |
| Year | 1990 |
| Title | Spectrophotometric Determination of Circulating Cholinesterases in Rats |
| Authors | Matthew, CB; Chapin, CL |
| Journal | Aviation |
| Volume | 61 |
| Issue | 4 |
| Page Numbers | 374-378 |
| Abstract | A spectrophotometric method for determining cholinesterase (ChE) activity in the whole blood of rats was developed. The method was based on a commercially available reagent set. The kit utilized acetylthiocholine which served as the substrate. The reaction between ChE in a blood sample produced thiocholine which was then reacted with dithiobisnitrobenzoic-acid to form 2-nitro-5-mercapto-benzoate (NMBZ). The absorbance of NMBZ was measured at 405 nanometers. NMBZ absorbance was directly proportional to the ChE activity in the sample after correcting for nonenzymatic hydrolysis of the substrate. Male Sprague-Dawley-rats were injected intravenously with 0, 50, 100, or 200 micrograms per kilogram physostigmine-salicylate (57647) (PHY). Blood samples were obtained from the lateral tail vein or from the jugular vein with a catheter 15 or 60 minutes after dosing and analyzed for ChE using the procedure. PHY induced dose dependent decreases in ChE activity. The extent of inhibition did not vary significantly between sampling sites; however, the extent of inhibition was less in samples analyzed 60 minutes after dosing compared with those obtained 15 minutes after dosing. Experiments leading to optimization of the procedure were described. The authors conclude that the spectrophotometric assay based on the commercial reagent kit can be used to determine ChE activity in rat blood. The blood samples can be taken from peripheral or central sites. |
| Pmid | 2339976 |
| Wosid | WOS:A1990CX52600016 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025292946&partnerID=40&md5=7a3da481d9a200e122ba0dd9f4b1fabe |
| Is Public | Yes |
| Language Text | English |
| Keyword | animal experiment; article; cholinesterase blood level; enzyme analysis; male; nonhuman; rat; spectrophotometry; Animal; Blood Specimen Collection; Cholinesterases; In Vitro; Rats; Spectrophotometry; Time Factors; Animalia |