Glutathione Transferases in Human Nasal Mucosa
Aceto, A; Di Ilio, C; Angelucci, S; Longo, V; Gervasi, PG; Federici, G
| HERO ID | 2741842 |
|---|---|
| In Press | No |
| Year | 1989 |
| Title | Glutathione Transferases in Human Nasal Mucosa |
| Authors | Aceto, A; Di Ilio, C; Angelucci, S; Longo, V; Gervasi, PG; Federici, G |
| Journal | Archives of Toxicology |
| Volume | 63 |
| Issue | 6 |
| Page Numbers | 427-431 |
| Abstract | Glutathione-transferase (GST) systems in human nasal mucosa were purified and characterized. Nasal membranes were removed from five males and five females during therapeutic surgery. Samples were minced, homogenized, and centrifuged to obtain supernatant fractions for GST assay and subsequent enzyme purification by affinity chromatography and isoelectric focusing. Characterization was performed by SDS/polyacrylamide gel electrophoresis (PAGE), Western blot analysis, and GST assay. GST activity for the ten samples in a 1-chloro-2,4-dinitrobenzene substrate ranged from 47 to 113 nanomoles per minute per milligram (nmol/min/mg) protein (mean, 76.8nmol/min/mg). Mean GST activity in Sprague-Dawley-rat nasal mucosa was 18.0nmol/min/mg protein. Pooled fractions passed through an affinity column increased in specific activity to 17 micromoles/min/mg protein. Isoelectric focusing indicated that 85 percent of GST activity was explained by an anionic peak at pH of 4.5, while remaining peaks were near neutral or basic; the acidic peak was identified as class pi GST. The affinity bound fraction contained a GST subunit which migrated at 28,000Mr, and could not be identified as either a pi, mu, or alpha isoenzyme. The authors suggest that this isoenzyme may be specific to nasal mucosal tissue. |
| Doi | 10.1007/BF00316443 |
| Pmid | 2619555 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024467867&doi=10.1007%2fBF00316443&partnerID=40&md5=3fccf7b09be725b98c34428820a90f89 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Glutathione transferase; Nasal mucosa |