Expression of Wnt5a during development of anorectal malformations in a rat model of prenatal exposure to di(n-butyl) phthalate
Li, EH; Han, BM; Sun, WL; Liang, SJ; Xia, SJ; Jiang, JT
HERO ID
2510762
Reference Type
Journal Article
Year
2014
Language
English
PMID
| HERO ID | 2510762 |
|---|---|
| In Press | No |
| Year | 2014 |
| Title | Expression of Wnt5a during development of anorectal malformations in a rat model of prenatal exposure to di(n-butyl) phthalate |
| Authors | Li, EH; Han, BM; Sun, WL; Liang, SJ; Xia, SJ; Jiang, JT |
| Journal | Toxicology Mechanisms and Methods |
| Volume | 24 |
| Issue | 7 |
| Page Numbers | 455-460 |
| Abstract | Mounting evidence has indicated the crucial role of Wnt5a in the embryonic development including guts. However, the Wnt5a involvement in the process of anorectal malformations (ARMs) remains unclear. In this study, we examined the expression of Wnt5a during ARMs development in the offspring of di(n-butyl) phthalate (DBP)-treated pregnant rats. During the neonatal period, Wnt5a expression was evaluated in the terminal rectum of ARM offspring, non-ARM littermates and controls. Using real-time polymerase chain reaction (real-time PCR), western-blot analysis and immunohistochemistry approaches, we found a significant decrease of Wnt5a expression in DBP-induced ARMs rats. Collectively, our results demonstrate the aberrant expression of Wnt5a during anorectal development, which suggests that Wnt5a might be involved in DBP-induced ARMs. |
| Doi | 10.3109/15376516.2014.932037 |
| Pmid | 24916655 |
| Wosid | WOS:000342235100002 |
| Url | https://search.proquest.com/docview/1563061680?accountid=171501 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Anorectal malformations; di(n-butyl) phthalate; Wnt5a |
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