Immobilization of glucose oxidase into a nanoporous TiO₂ film layered on metallophthalocyanine modified vertically-aligned carbon nanotubes for efficient direct electron transfer
Cui, HF; Zhang, K; Zhang, YF; Sun, YL; Wang, J; Zhang, WD; Luong, JH
| HERO ID | 2339421 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | Immobilization of glucose oxidase into a nanoporous TiO₂ film layered on metallophthalocyanine modified vertically-aligned carbon nanotubes for efficient direct electron transfer |
| Authors | Cui, HF; Zhang, K; Zhang, YF; Sun, YL; Wang, J; Zhang, WD; Luong, JH |
| Journal | Biosensors and Bioelectronics |
| Volume | 46 |
| Page Numbers | 113-118 |
| Abstract | Glucose oxidase (GOD) was adsorbed into a nanoporous TiO₂ film layered on the surface of an iron phthalocyanine (FePc) vertically-aligned carbon nanotube (CNT) modified electrode. A Nafion film was then dropcast on the electrode's surface to improve operational and storage stabilities of the GOD-based electrode. Scanning electron microscopy (SEM) micrographs revealed the formation of FePc and nanoporous TiO₂ nanoparticles along the sidewall and the tip of CNTs. Cyclic voltammograms of the GOD electrode in neutral PBS exhibited a pair of well-defined redox peaks, attesting the direct electron transfer of GOD (FAD/FADH₂) with the underlying electrode. The potential of glucose electro-oxidation under nitrogen was ∼+0.12 V with an oxidation current density of 65.3 μA cm(-2) at +0.77 V. Voltammetric and amperometric responses were virtually unaffected by oxygen, illustrating an efficient and fast direct electron transfer. The modification of the CNT surface with FePc resulted in a biosensor with remarkable detection sensitivity with an oxygen-independent bioelectrocatalysis. In deaerated PBS, the biosensor displayed average response time of 12 s, linearity from 50 μM to 4 mM, and a detection limit of 30 μM (S/N=3) for glucose. |
| Doi | 10.1016/j.bios.2013.02.029 |
| Pmid | 23517827 |
| Wosid | WOS:000318325200019 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: Biosensors & bioelectronics ISSN: 1873-4235 |
| Is Public | Yes |
| Language Text | English |