A method for the determination of environmental contaminants in living marine mammals using microscale samples of blubber and blood
Newman, JW; Vedder, JM; Jarman, WM; Chang, RR
HERO ID
2329466
Reference Type
Journal Article
Year
1994
Language
English
PMID
| HERO ID | 2329466 |
|---|---|
| In Press | No |
| Year | 1994 |
| Title | A method for the determination of environmental contaminants in living marine mammals using microscale samples of blubber and blood |
| Authors | Newman, JW; Vedder, JM; Jarman, WM; Chang, RR |
| Journal | Chemosphere |
| Volume | 29 |
| Issue | 4 |
| Page Numbers | 671-681 |
| Abstract | As part of a study examining the possible effects of organochlorine compounds on juvenile northern elephant seals (Mirounga angustirostris), blubber and blood samples were taken from animals present on the Ario Nuevo (California) rookery, and from animals admitted for rehabilitation at The Marine Mammal Center (Sausalito, CA). Blubber samples were collected from immobilized seals. A pre-cleaned 6mm K-medic biopsy punch was used to extract the blubber from a 1 cm incision near the hip, near the dorsal mid point. Blood samples were taken from the extradural vein; two mL of serum was analyzed for organochlorine compounds. Blubber samples (approximately 0.1g) were ground with Na2SO4 and extracted with 20 mL hexane:methylene chloride (1:1). Sera samples were extracted using commercial available disposable C18 columns. The extracts were separate on a micro-Florisil column, and analyzed by HRGC-ECD. Lipid determination in the serum was obtained colorimetric analysis with 20 mu |
| Doi | 10.1016/0045-6535(94)90038-8 |
| Pmid | 7922154 |
| Wosid | WOS:A1994PE85100004 |
| Url | https://linkinghub.elsevier.com/retrieve/pii/0045653594900272 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Relationship(s) |
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