Measurement of stable isotopic enrichment and concentration of long-chain fatty acyl-carnitines in tissue by HPLC-MS
Sun, D; Cree, MG; Zhang, XJ; Bøersheim, E; Wolfe, RR
HERO ID
2325522
Reference Type
Journal Article
Year
2006
Language
English
PMID
| HERO ID | 2325522 |
|---|---|
| In Press | No |
| Year | 2006 |
| Title | Measurement of stable isotopic enrichment and concentration of long-chain fatty acyl-carnitines in tissue by HPLC-MS |
| Authors | Sun, D; Cree, MG; Zhang, XJ; Bøersheim, E; Wolfe, RR |
| Journal | Journal of Lipid Research |
| Volume | 47 |
| Issue | 2 |
| Page Numbers | 431-439 |
| Abstract | We have developed a new method for the simultaneous measurements of stable isotopic tracer enrichments and concentrations of individual long-chain fatty acyl-carnitines in muscle tissue using ion-pairing high-performance liquid chromatography-electrospray ionization quadrupole mass spectrometry in the selected ion monitoring (SIM) mode. Long-chain fatty acyl-carnitines were extracted from frozen muscle tissue samples by acetonitrile/methanol. Baseline separation was achieved by reverse-phase HPLC in the presence of the volatile ion-pairing reagent heptafluorobutyric acid. The SIM capability of a single quadrupole mass analyzer allows further separation of the ions of interest from the sample matrixes, providing very clean total and selected ion chromatograms that can be used to calculate the stable isotopic tracer enrichment and concentration of long-chain fatty acyl-carnitines in a single analysis. The combination of these two separation techniques greatly simplifies the sample preparation procedure and increases the detection sensitivity. Applying this protocol to biological muscle samples proves it to be a very sensitive, accurate, and precise analytical tool. |
| Doi | 10.1194/jlr.D500026-JLR200 |
| Pmid | 16301738 |
| Wosid | WOS:000234844700020 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-33244485451&doi=10.1194%2fjlr.D500026-JLR200&partnerID=40&md5=7f9d728706acf670246cfdd5abc7c118 |
| Is Public | Yes |
| Language Text | English |
| Keyword | High-performance liquid chromatography-mass spectrometry; Ion-pairing high-performance liquid chromatography; Muscle tissue |