Purification of Two Triterpenoids from Schisandra chinensis by Macroporous Resin Combined with High-Speed Counter-Current Chromatography
Zhu, L; Li, B; Liu, X; Meng, X
HERO ID
2283827
Reference Type
Journal Article
Year
2014
Language
English
PMID
| HERO ID | 2283827 |
|---|---|
| In Press | No |
| Year | 2014 |
| Title | Purification of Two Triterpenoids from Schisandra chinensis by Macroporous Resin Combined with High-Speed Counter-Current Chromatography |
| Authors | Zhu, L; Li, B; Liu, X; Meng, X |
| Journal | Journal of Chromatographic Science |
| Volume | 52 |
| Issue | 9 |
| Page Numbers | 1082-1088 |
| Abstract | A method for preparative purification of corosolic acid and nigranoic acid from Schisandra chinensis (SC) was established using a combination of macroporous absorption resin column separation and high-speed counter-current chromatography (HSCCC). The crude extracts obtained from SC using 70% ethanol were separated on a macroporous resin column and then eluted with a graded ethanol series. The 70% ethanol fraction was used as the sample for separation of the two triterpenoids by HSCCC. The two-phase solvent system used for HSCCC separation was chloroform-n-butanol-methanol-water (10:0.5:7:4, v/v/v/v). The upper phase was used as the stationary phase of HSCCC. Corosolic acid (16.4 mg) of 96.3% purity and nigranoic acid (9.5 mg) of 98.9% purity were obtained in a one-step HSCCC separation from 100 mg of the sample. The structures of corosolic acid and nigranoic acid were identified by (1)H-nuclear magnetic resonance (NMR) and (13)C-NMR. |
| Doi | 10.1093/chromsci/bmt168 |
| Pmid | 24220992 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |