Effects of lipoic acid and dihydrolipoic acid on 4-aminophenol-mediated erythrocytic toxicity in vitro
Coleman, MD; Williams, C; Haenen, GRMM
HERO ID
2277894
Reference Type
Journal Article
Year
2006
Language
English
PMID
| HERO ID | 2277894 |
|---|---|
| In Press | No |
| Year | 2006 |
| Title | Effects of lipoic acid and dihydrolipoic acid on 4-aminophenol-mediated erythrocytic toxicity in vitro |
| Authors | Coleman, MD; Williams, C; Haenen, GRMM |
| Journal | Basic & Clinical Pharmacology & Toxicology |
| Volume | 99 |
| Issue | 3 |
| Page Numbers | 225-229 |
| Abstract | The effects of the antioxidant lipoic acid and its reduced form, dihydrolipoic acid (DHLA), were studied on the process of the erythrocytic toxicity of 4-aminophenol in human erythrocytes in vitro. 4-Aminophenol alone caused a stepwise increase in methaemoglobin formation, along with a commensurate decrease in total thiols. At 10 min., in the presence of lipoic acid alone and the thiol depletor 1-chloro-2,4-dinitrobenzene (CDNB) alone, 4-aminophenol-mediated methaemoglobin formation was significantly increased, whilst thiol levels were significantly reduced compared with the 4-aminophenol alone. At 10 min., with DHLA and CDNB alone, 4-aminophenol was associated with significantly increased methaemoglobin formation. However, thiol levels were not significantly different in the presence of DHLA compared with 4-aminophenol alone, although thiol levels were different compared with control (4-aminophenol alone) in the incubations with CDNB alone. At 15 min., only CDNB/4-aminophenol methaemoglobin formation differed from control, whilst thiol levels were significantly lower in the presence of CDNB alone compared with 4-aminophenol alone. Lipoic acid enhanced the toxicity of 4-aminophenol in terms of increased methaemoglobin formation coupled with increased thiol depletion, whilst DHLA showed increased 4-aminophenol-mediated methaemoglobin formation without thiol depletion. Lipoic acid, and to a lesser extent its reduced derivative DHLA, acted as a prooxidant in the presence of 4-aminophenol, enhancing the oxidative stress effects of the amine in human erythrocytes. |
| Doi | 10.1111/j.1742-7843.2006.pto_499.x |
| Pmid | 16930295 |
| Wosid | WOS:000240006800006 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-33747884945&doi=10.1111%2fj.1742-7843.2006.pto_499.x&partnerID=40&md5=59ce94676e6d70d0476a0565d12f7a1c |
| Is Public | Yes |
| Language Text | English |