Synthesis, DNA affinity, and Antiprotozoal activity of fused ring dicationic compounds and their prodrugs

Arafa, RK; Brun, R; Wenzler, T; Tanious, FA; Wilson, WD; Stephens, CE; Boykin, DW

HERO ID

2243359

Reference Type

Journal Article

Year

2005

Language

English

PMID

16107146

HERO ID 2243359
In Press No
Year 2005
Title Synthesis, DNA affinity, and Antiprotozoal activity of fused ring dicationic compounds and their prodrugs
Authors Arafa, RK; Brun, R; Wenzler, T; Tanious, FA; Wilson, WD; Stephens, CE; Boykin, DW
Journal Journal of Medicinal Chemistry
Volume 48
Issue 17
Page Numbers 5480-5488
Abstract Dicationic guanidine, N-alkylguanidine, and reversed amidine derivatives of fused ring systems have been synthesized from their corresponding bis-amines. DNA binding studies suggest that the diguanidines and the N-alkyl diguanidines fluorenes bind in the minor groove in a manner similar to that of the previously reported dicationic carbazole derivatives. The diguanidines and the N-alkyl diguanidines showed promising in vitro activity against both Trypanosoma brucei rhodesiense and Plasmodium falciparum. Promising in vivo biological results were obtained for the dicationic N-isopropylguanidino-9H-fluorene, giving 4/4 cures of the treated animals in the STIB900 animal model for African trypanosomiasis. The N-methyl analogue showed high activity as well. In addition, with the goal of enhancing the oral bioavailability, two novel classes of potential guanidine prodrugs were prepared. The N-alkoxyguanidine derivatives were not effective as prodrugs. In contrast, a number of the carbamates showed promising activity. The value of the carbamate prodrugs was clearly demonstrated by the results, which gave 4/4 cures on oral administration in the STIB900 mouse model.
Doi 10.1021/jm058190h
Pmid 16107146
Wosid WOS:000231459600011
Is Certified Translation No
Dupe Override No
Comments Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-23944453324&doi=10.1021%2fjm058190h&partnerID=40&md5=6d52258d3554b87b002075e767030ff2
Is Public Yes
Language Text English
Keyword 2,7 bis(n' ethoxycarbonyl n'' isopropyl)guanidino 9h fluorene; 2,7 bis(n' ethoxycarbonyl)guanidino 9h fluorene; 2,7 bis(n' ethoxycarbonyl)guanidinofluoren 9 one; 2,7 bis(n' ethyl)guanidino 9h fluorene; 2,7 bis(n' isopropyl)guanidino 9h fluorene; 2,7 bis(n' methyl)guanidino 9h fluorene; 2,7 bis[4 (iminobenzylamino)] 9h fluorene; 2,7 bis[4 [imino (2 pyridylmethyl)]amino] 9h fluorene; 2,7 bisguanidino 9h fluorene; 2,7 bisguanidinoanthraquinone; 2,7 bisguanidinofluoren 9 one; 2,7 nis(n ethoxycarbonyl n'' methyl)guanidino 9h fluorene; 3,6 bisguanidinoacridine; amidine; guanidine derivative; prodrug; unclassified drug; African trypanosomiasis; animal experiment; animal model; antiprotozoal activity; article; controlled study; drug binding; drug bioavailability; drug DNA binding; drug efficacy; drug structure; drug synthesis; in vitro study; in vivo study; mouse; nonhuman; Plasmodium falciparum; structure activity relation; Trypanosoma rhodesiense; Animals; Antiprotozoal Agents; Carbamates; Cations; Circular Dichroism; DNA; Fluorenes; Guanidines; Mice; Plasmodium falciparum; Poly dA-dT; Prodrugs; Structure-Activity Relationship; Trypanosoma brucei rhodesiense; Trypanosomiasis, African