Nitrogen isotope effects induced by anammox bacteria
Brunner, B; Contreras, S; Lehmann, MF; Matantseva, O; Rollog, M; Kalvelage, T; Klockgether, G; Lavik, G; Jetten, MS; Kartal, B; Kuypers, MM
HERO ID
2232984
Reference Type
Journal Article
Year
2013
Language
English
PMID
| HERO ID | 2232984 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | Nitrogen isotope effects induced by anammox bacteria |
| Authors | Brunner, B; Contreras, S; Lehmann, MF; Matantseva, O; Rollog, M; Kalvelage, T; Klockgether, G; Lavik, G; Jetten, MS; Kartal, B; Kuypers, MM |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 110 |
| Issue | 47 |
| Page Numbers | 18994-18999 |
| Abstract | Nitrogen (N) isotope ratios ((15)N/(14)N) provide integrative constraints on the N inventory of the modern ocean. Anaerobic ammonium oxidation (anammox), which converts ammonium and nitrite to dinitrogen gas (N2) and nitrate, is an important fixed N sink in marine ecosystems. We studied the so far unknown N isotope effects of anammox in batch culture experiments. Anammox preferentially removes (14)N from the ammonium pool with an isotope effect of +23.5‰ to +29.1‰, depending on factors controlling reversibility. The N isotope effects during the conversion of nitrite to N2 and nitrate are (i) inverse kinetic N isotope fractionation associated with the oxidation of nitrite to nitrate (-31.1 ± 3.9‰), (ii) normal kinetic N isotope fractionation during the reduction of nitrite to N2 (+16.0 ± 4.5‰), and (iii) an equilibrium N isotope effect between nitrate and nitrite (-60.5 ± 1.0‰), induced when anammox is exposed to environmental stress, leading to the superposition of N isotope exchange effects upon kinetic N isotope fractionation. Our findings indicate that anammox may be responsible for the unresolved large N isotope offsets between nitrate and nitrite in oceanic oxygen minimum zones. Irrespective of the extent of N isotope exchange between nitrate and nitrite, N removed from the combined nitrite and nitrate (NOx) pool is depleted in (15)N relative to NOx. This net N isotope effect by anammox is superimposed on the N isotope fractionation by the co-occurring reduction of nitrate to nitrite in suboxic waters, possibly enhancing the overall N isotope effect for N loss from oxygen minimum zones. |
| Doi | 10.1073/pnas.1310488110 |
| Pmid | 24191043 |
| Wosid | WOS:000327100600068 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |