Copper control of bacterial nitrous oxide emission and its impact on vitamin B12-dependent metabolism
Sullivan, MJ; Gates, AJ; Appia-Ayme, C; Rowley, G; Richardson, DJ
HERO ID
2232551
Reference Type
Journal Article
Year
2013
Language
English
PMID
| HERO ID | 2232551 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | Copper control of bacterial nitrous oxide emission and its impact on vitamin B12-dependent metabolism |
| Authors | Sullivan, MJ; Gates, AJ; Appia-Ayme, C; Rowley, G; Richardson, DJ |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 110 |
| Issue | 49 |
| Page Numbers | 19926-19931 |
| Abstract | Global agricultural emissions of the greenhouse gas nitrous oxide (N2O) have increased by around 20% over the last 100 y, but regulation of these emissions and their impact on bacterial cellular metabolism are poorly understood. Denitrifying bacteria convert nitrate in soils to inert di-nitrogen gas (N2) via N2O and the biochemistry of this process has been studied extensively in Paracoccus denitrificans. Here we demonstrate that expression of the gene encoding the nitrous oxide reductase (NosZ), which converts N2O to N2, is regulated in response to the extracellular copper concentration. We show that elevated levels of N2O released as a consequence of decreased cellular NosZ activity lead to the bacterium switching from vitamin B12-dependent to vitamin B12-independent biosynthetic pathways, through the transcriptional modulation of genes controlled by vitamin B12 riboswitches. This inhibitory effect of N2O can be rescued by addition of exogenous vitamin B12. |
| Doi | 10.1073/pnas.1314529110 |
| Pmid | 24248380 |
| Wosid | WOS:000327744900063 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | denitrification; transcription; NosR; NosC |