E-assay concept: detection of bisphenol A with a label-free electrochemical competitive immunoassay
Wang, X; Reisberg, S; Serradji, N; Anquetin, G; Pham, MC; Wu, W; Dong, CZ; Piro, B
| HERO ID | 2219816 |
|---|---|
| In Press | No |
| Year | 2014 |
| Title | E-assay concept: detection of bisphenol A with a label-free electrochemical competitive immunoassay |
| Authors | Wang, X; Reisberg, S; Serradji, N; Anquetin, G; Pham, MC; Wu, W; Dong, CZ; Piro, B |
| Journal | Biosensors and Bioelectronics |
| Volume | 53 |
| Page Numbers | 214-219 |
| Abstract | A label-free electrochemical immunosensor was developed by electropolymerization of N-(3-(4-(2-(4-hydroxyphenyl)propan-2-yl)phenoxy)propyl) 3-(5-hydroxy-1,4-dihydro-1,4-dioxonaphthalen-2(3)-yl)propionamide (JugBPA). By combination with an antibody directed to bisphenol A (αBPA), this conducting polymer-based biosensor can detect BPA directly with a limit of detection of 2pgmL(-1). Square wave voltammetry shows that the polymer film presents a current decrease upon anti-BPA binding and an opposite current increase upon BPA addition in solution. This electrochemical immunosensor (E-assay) also shows high selectivity towards closely related compounds (bisphenol A dimethacrylate, and dibutyl phthalate). The E-assay concept described here could be a promising tool for simple, low-cost and reagentless on-site environmental monitoring. |
| Doi | 10.1016/j.bios.2013.09.062 |
| Pmid | 24140871 |
| Wosid | WOS:000329881100034 |
| Url | https://search.proquest.com/docview/2000219408?accountid=171501 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: Biosensors & bioelectronics ISSN: 1873-4235 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Competitive immunoassay; Bisphenol A detection; Label-free detection; Electrochemical immunosensor |