Perfluorooctane sulfonamide-mediated modulation of hepatocellular lipid homeostasis and oxidative stress responses in Atlantic Salmon Hepatocytes
Wågbø, A; Cangialosi, MV; Cicero, N; Letcher, RJ; Arukwe, A
HERO ID
2187308
Reference Type
Journal Article
Year
2012
Language
English
PMID
| HERO ID | 2187308 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | Perfluorooctane sulfonamide-mediated modulation of hepatocellular lipid homeostasis and oxidative stress responses in Atlantic Salmon Hepatocytes |
| Authors | Wågbø, A; Cangialosi, MV; Cicero, N; Letcher, RJ; Arukwe, A |
| Journal | Chemical Research in Toxicology |
| Volume | 25 |
| Issue | 6 |
| Page Numbers | 1253-1264 |
| Abstract | We have investigated the effects of perfluorooctane sulfonamide (PFOSA) on cellular functions and lipid homeostasis (including beta-oxidation) in salmon primary hepatocytes. Salmon hepatocytes were exposed to PFOSA at 0 (control), 2, 20, and 50 mu M for 12 and 24 h. Fatty acids (FM) and lipids were determined by GC-MS; FA elongase (FAE), Delta 5-desaturase (FADS), Delta 6-desaturase (FAD6), peroxisome proliferator-activated receptors (PPARs), acyl coenzyme A (ACOX-1), glutathione peroxidase (GPx), catalase (CAT), and glutathione S-transferase (GST) mRNA were analyzed using qPCR. GST activity was analyzed by biochemical assays using 1-chloro-2,4-dinitrobenzene (CDNB) as substrate. Our data showed that PFOSA produced significant changes in FA composition that predominantly involved a decrease (at 12 h) and an increase (at 24 h) in FA methyl esters (FAMEs), MUFA, total PUFA, and (n-3 and n-6) PUFA. Particularly, an increase of alpha-linolenic acid (ALA; 18:3n-3), eicosapentaenoic acid [EPA; 20:5n-3], and arachidonic acid [ARA: 20:4n-6] with associated increase in FAE, FADS, and FAD6 mRNA were observed after PFOSA exposure, while cis-13,16-docosadienoic acid (22:2) was significantly decreased. PFOSA produced apparent concentration-dependent increase of PPAR alpha and PPAR gamma. CAT, GPx, and GST mRNA show that PFOSA produced concentration- and time-specific increase of CAT and GST, but no changes in GST activity were observed. In general, these responses indicate that PFOSA evokes deleterious effects on cellular lipid homeostasis a;ad transcriptional responses that regulate cellular oxidative homeostasis in salmon hepatocytes. |
| Doi | 10.1021/tx300110u |
| Pmid | 22594583 |
| Wosid | WOS:000305300100011 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84862518194&doi=10.1021%2ftx300110u&partnerID=40&md5=6734c04339d31640e2d006d053fb0d72 |
| Is Public | Yes |
| Language Text | English |