Metabolic changes in frozen-clamped livers of rats caused by ingestion of polychlorinated biphenyls
Mehlman, MA; Yin, L; Nielsen, RC
HERO ID
2176147
Reference Type
Journal Article
Year
1974
Language
English
PMID
| HERO ID | 2176147 |
|---|---|
| In Press | No |
| Year | 1974 |
| Title | Metabolic changes in frozen-clamped livers of rats caused by ingestion of polychlorinated biphenyls |
| Authors | Mehlman, MA; Yin, L; Nielsen, RC |
| Journal | Toxicology and Applied Pharmacology |
| Volume | 27 |
| Issue | 2 |
| Page Numbers | 300-307 |
| Abstract | Polychlorinated biphenyls (PCB environmental and food contaminants) were administered for 12 days in the diet as Aroclor 1254 to male Osborne-Mendel rats at concentrations of 0, 75 or 400 ppm, and effects on body weight gain, food consumption and adipose tissue weights were determined. Concentrations of intermediary metabolites in the liver were also determined; cytoplasmic and mitochondrial redox states and cytoplasmic phosphorylation states were calculated from these concentrations. Rats given 400 ppm of Aroclor 1254 had significantly decreased body weight gains and adipose tissue weights with respect to controls; rats given 75 ppm showed no significant differences in these parameters. Significant differences in the concentrations of intermediary metabolites were found in the livers of rats fed Aroclor 1254 at both the 75 and 400 ppm levels. These concentration changes resembled those occurring as a consequence of thiamine deficiency. The cytoplasmic redox state of liver was greatly shifted toward a highly oxidized condition, as evidenced by significantly increased (NADP+): ratios, in rats receiving Aroclor at 400 ppm; this effect was less pronounced in rats receiving 75 ppm. A similar increase in (NADP+): in the cytosol had been observed in livers from thiamine-deficient rats. No significant differences could be observed in the liver cytoplasmic (NAD+): ratio with respect to control values in either experimental group. The mitochondrial (NAD+): ratio was significantly increased in the group given 400 ppm. The phosphorylation state of liver cytoplasm was significantly increased in rats receiving 400 ppm of Aroclor, and somewhat less in rats receiving 75 ppm. |
| Doi | 10.1016/0041-008X(74)90201-4 |
| Pmid | 4152956 |
| Wosid | WOS:A1974S363000010 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Relationship(s) |
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