Inhibition of rat testicular androgenesis by a polychlorinated biphenyl mixture Aroclor 1248
Andric, SA; Kostic, TS; Stojilkovic, SS; Kovacevic, RZ
HERO ID
2152151
Reference Type
Journal Article
Year
2000
Language
English
PMID
| HERO ID | 2152151 |
|---|---|
| In Press | No |
| Year | 2000 |
| Title | Inhibition of rat testicular androgenesis by a polychlorinated biphenyl mixture Aroclor 1248 |
| Authors | Andric, SA; Kostic, TS; Stojilkovic, SS; Kovacevic, RZ |
| Journal | Biology of Reproduction |
| Volume | 62 |
| Issue | 6 |
| Page Numbers | 1882-1888 |
| Abstract | Polychlorinated biphenyls (PCBs) are complex mixtures of congeners that exhibit carcinogenic and toxicant activities in a variety of mammalian tissues. Here, we studied the acute in vivo and in vitro effects of a commercially used PCB product, Aroclor 1248 (A1248), a mixture of tri-, tetra-, and pentachloro congeners. Single intraperitoneal (i.p.) or bilateral intratesticular (i.t.) injections of A1248 decreased serum androgen levels in both groups 24 h after injection. Chorionic gonadotropin-stimulated androgen production by acute testicular cultures from both groups was also reduced, and progesterone production was attenuated in cultures from i.t.-treated animals. The capacity of the postmitochondrial fractions from testes of i.t.-treated animals to convert pregnenolone to progesterone and progesterone to testosterone was reduced as well. In vitro studies revealed that a 10- to 15-min exposure of postmitochondrial testicular fractions and intact interstitial cells from normal animals to A1248 in a subnanomolar concentration range was sufficient to attenuate the conversion of pregnenolone to progesterone and progesterone to testosterone. At micromolar concentrations, A1248 added in vitro also inhibited the conversion of Delta(4)-androstendione to testosterone without affecting the viability of interstitial cells. These results indicate that A1248 down-regulates the testicular androgenesis by an acute inhibition of 3beta-hydroxysteroid dehydrogenase, 17alpha-hydroxylase/lyase, and 17beta-hydroxysteroid dehydrogenase activities. |
| Doi | 10.1095/biolreprod62.6.1882 |
| Pmid | 10819796 |
| Wosid | WOS:000087239600051 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |