Differential induction of cytochrome-p-450-dependent monooxygenase, epoxide hydrolase, glutathione transferase and udp glucuronosyl transferase activities in the liver of the rainbow-trout by beta-naphthoflavone or clophen-a50
Andersson, T; Pesonen, M; Johansson, C
HERO ID
2152105
Reference Type
Journal Article
Year
1985
Language
English
PMID
| HERO ID | 2152105 |
|---|---|
| In Press | No |
| Year | 1985 |
| Title | Differential induction of cytochrome-p-450-dependent monooxygenase, epoxide hydrolase, glutathione transferase and udp glucuronosyl transferase activities in the liver of the rainbow-trout by beta-naphthoflavone or clophen-a50 |
| Authors | Andersson, T; Pesonen, M; Johansson, C |
| Journal | Biochemical Pharmacology |
| Volume | 34 |
| Issue | 18 |
| Page Numbers | 3309-3314 |
| Abstract | After administration of beta-naphthoflavone and Clophen A50 to juvenile rainbow trout, activities of hepatic cytochrome P-450-dependent deethylation of 7-ethoxyresorufin was increased 172- and 49-fold, respectively. Glutathione transferase activity towards 1-chloro 2,4 dinitrobenzene and UDP glucuronosyltransferase activities towards p-nitrophenol, 1-naphthol and testosterone were increased 1.4 to 3.0-fold by beta-naphthoflavone or Clophen A50. However, significant increases of the rate of glucuronidation of 1-naphthol by Clophen A50 and of testosterone by both Clophen A50 and beta-naphthoflavone were only determined when the activities were measured in digitonin activated microsomes. Epoxide hydrolase activity was not affected by beta-naphthoflavone or Clophen A50. The time course of induction of the various xenobiotic metabolizing enzymes exhibited different patterns. 7-Ethoxyresorufin-O-deethylase activity reached peak values 3 and 7 days after the administration of beta-naphthoflavone and Clophen A50, respectively. The rate of induction of glutathione transferase activity and UDP glucuronosyltransferase activities towards p-nitrophenol and 1-naphthol were relatively slow and did not reach distinct peak levels. These activities were still on maximum levels 4-6 weeks after the treatment. Glucuronidation of testosterone reached peak values 1 week after treatment with both beta-naphthoflavone and Clophen A50. The dissimilar patterns of induction of the cytochrome P-450-dependent activities and the various conjugation activities may indicate that these xenobiotic metabolizing enzymes are differently regulated in the rainbow trout liver. |
| Doi | 10.1016/0006-2952(85)90351-X |
| Pmid | 3929791 |
| Wosid | WOS:A1985AQV7800015 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022270572&doi=10.1016%2f0006-2952%2885%2990351-X&partnerID=40&md5=24ba144440d6bea3017b85203dbee3ec |
| Is Public | Yes |
| Language Text | English |