Suppression of cell proliferation, induction of apoptosis and cell cycle arrest: Chemopreventive activity of vanadium in vivo and in vitro
Ray, RS; Ghosh, B; Rana, A; Chatterjee, M
HERO ID
2124023
Reference Type
Journal Article
Year
2007
Language
English
PMID
| HERO ID | 2124023 |
|---|---|
| In Press | No |
| Year | 2007 |
| Title | Suppression of cell proliferation, induction of apoptosis and cell cycle arrest: Chemopreventive activity of vanadium in vivo and in vitro |
| Authors | Ray, RS; Ghosh, B; Rana, A; Chatterjee, M |
| Journal | International Journal of Cancer |
| Volume | 120 |
| Issue | 1 |
| Page Numbers | 13-23 |
| Abstract | In the present study, the authors evaluated the anticancer mechanism of vanadium, a dietary micronutrient and an important pharmacological agent, on a defined model of chemically induced rat mammary carcinogenesis in vivo and on human breast cancer cell line MCF7 in vitro. Female Sprague-Dawley rats were treated with 7,12-dimethylbenz(alpha)anthracene (0.5 mg/100 g body weight) by a single tail vein injection in an oil emulsion to induce mammary preneoplasia. Vanadium (ammonium monovanadate) at a concentration of 0.5 ppm (4.27 mu mol/l) was supplemented in drinking water and given ad libitum to the experimental groups for 24 weeks. Histological finding showed substantial repair of hyperplastic lesions. There was a significant reduction in incidence, multiplicity (34%, p < 0.01), size of palpable mammary tumors and delay in mean latency period of tumor appearance. Immunohistochemical analysis in vivo indicated a decrease in cell proliferation (24.68% p < 0.05) and an increase among the TUNEL-positive apoptotic cells along with strong expressions of p53 and Bax, and downregulation of Bcl2 proteins in the mammary tissue of vanadium-treated animals. Further, MCF7 cells were cultured in minimal essential medium and were treated with 100, 175 and 250 mu M of vanadium (ammonium monovanadate) for 36 hr. Exposure of MCF7 cells to vanadium led to induction of apoptosis in a dose-dependent manner. It was found further that vanadium treatment brought about a prominent cell cycle arrest and chromosomal condensation, leading to apoptosis (42.62 %, p < 0.05). Results of both the in vivo and in vitro study demonstrate that vanadium has the potential to be developed into an anti-breast cancer drug in the near future. (c) 2006 Wiley-Liss, Inc. |
| Doi | 10.1002/ijc.22277 |
| Pmid | 17058199 |
| Wosid | WOS:000242653300003 |
| Url | https://www.proquest.com/docview/68363923?accountid=171501&bdid=13857&_bd=GBtGhhxcsrxKx2oOO0ggMrBVgtg%3D |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | mammary carcinogenesis; cell proliferation; cell regulation; MCF7; cell cycle; apoptosis; vanadium |