Cell-Free Biosystems for Biomanufacturing
You, C; Zhang, YHP
| HERO ID | 2114236 |
|---|---|
| Material Type | Review |
| In Press | No |
| Year | 2013 |
| Title | Cell-Free Biosystems for Biomanufacturing |
| Authors | You, C; Zhang, YHP |
| Journal | Advances in Biochemical Engineering. Biotechnology |
| Volume | 131 |
| Page Numbers | 89-119 |
| Abstract | Although cell-free biosystems have been used as a tool for investigating fundamental aspects of biological systems for more than 100 years, they are becoming an emerging biomanufacturing platform in the production of low-value biocommodities (e.g., H(2), ethanol, and isobutanol), fine chemicals, and high-value protein and carbohydrate drugs and their precursors. Here we would like to define the cell-free biosystems containing more than three catalytic components in a single reaction vessel, which although different from one-, two-, or three-enzyme biocatalysis can be regarded as a straightforward extension of multienzymatic biocatalysis. In this chapter, we compare the advantages and disadvantages of cell-free biosystems versus living organisms, briefly review the history of cell-free biosystems, highlight a few examples, analyze any remaining obstacles to the scale-up of cell-free biosystems, and suggest potential solutions. Cell-free biosystems could become a disruptive technology to microbial fermentation, especially in the production of high-impact low-value biocommodities mainly due to the very high product yields and potentially low production costs. |
| Doi | 10.1007/10_2012_159 |
| Pmid | 23111502 |
| Wosid | WOS:000321107000005 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Biocommodity engineering; Bioeconomy; Biofuels; Biomanufacturing; Cascade enzyme biocatalyst; Cell-free synthetic biology; Synthetic pathway biotransformation |