Effect of sulfur sources on specific desulfurization activity of Rhodococcus erythropolis KA2-5-1 in exponential fed-batch culture
Konishi, M; Kishimoto, K; Omasa, T; Katakura, Y; Shioya, S; Ohtake, H
| HERO ID | 2092128 |
|---|---|
| In Press | No |
| Year | 2005 |
| Title | Effect of sulfur sources on specific desulfurization activity of Rhodococcus erythropolis KA2-5-1 in exponential fed-batch culture |
| Authors | Konishi, M; Kishimoto, K; Omasa, T; Katakura, Y; Shioya, S; Ohtake, H |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 99 |
| Issue | 3 |
| Page Numbers | 259-263 |
| Abstract | The effects of sulfur sources on the desulfurization activity of Rhodococcus erythropolis KA2-5-1 were investigated by using an exponential fed-batch culture technique. The feed rate of a sulfur source was controlled independently of the feed rate of ethanol, which was used as a carbon and energy source. Among the sulfur sources examined were dibenzothiophene (DBT), ammonium sulfate, L-cysteine, L-methionine, and 2-amino-ethanesulfonic acid. When the fed-medium contained DBT as the sole sulfur source, KA2-5-1 cells showed a maximum desulfurization activity of approximately 130 mmol 2-HBP kg-cell(-1) h(-1). Similar levels of enzyme activity were also achieved with inexpensive ammonium sulfate by using the exponential fed-batch culture technique. In addition, higher levels of desulfurization activity were achieved by increasing the dosage of the DBT desulfurization (dsz) operon and dszD gene in R. erythropolis KA2-5-1. The recombinant strain showed a maximum desulfurization activity of approximately 250 mmol 2-HBP kg-cell(-1) h(-1) in the exponential fed-batch cultures. |
| Doi | 10.1263/jbb.99.259 |
| Pmid | 16233786 |
| Wosid | WOS:000229052300010 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | biodesulfurization; fed-batch culture; Rhodococcus erythropolis |