Expression of Matrix Metalloproteinases, Tissue Inhibitors of Metalloproteinases, and Extracellular Matrix mRNA Following Exposure to Mineral Fibers and Cigarette Smoke In Vivo
Morimoto, Y; Tsuda, T; Nakamura, H; Hori, H; Yamato, H; Nagata, N; Higashi, T; Kido, M; Tanaka, I
HERO ID
2072722
Reference Type
Journal Article
Year
1997
Language
English
PMID
| HERO ID | 2072722 |
|---|---|
| In Press | No |
| Year | 1997 |
| Title | Expression of Matrix Metalloproteinases, Tissue Inhibitors of Metalloproteinases, and Extracellular Matrix mRNA Following Exposure to Mineral Fibers and Cigarette Smoke In Vivo |
| Authors | Morimoto, Y; Tsuda, T; Nakamura, H; Hori, H; Yamato, H; Nagata, N; Higashi, T; Kido, M; Tanaka, I |
| Journal | Environmental Health Perspectives |
| Volume | 105 |
| Issue | SUPPL. 5 |
| Page Numbers | 1247-1251 |
| Abstract | Induction of matrix metalloproteinase, tissue inhibitors of metalloproteinase, and extracellular matrix mRNA expression by mineral fibers and cigarette smoke was studied in rats. Male Wistar-rats were instilled intratracheally with 2 milligrams of chrysotile (12001295) or aluminum-silicate (12141467) ceramic fibers (RCFs) after which they were sham exposed or exposed to sidestream cigarette smoke 5 days per week for 4 weeks. After exposure the rats were killed and their lungs removed. Some lungs were examined for histological changes. The RNA was extracted from other lungs and analyzed for mRNA coding for matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-2 (MMP-2), tissue inhibitors of MMP-1 (TIMP-1) and MMP-2 (TIMP-2), and type-I and type-IV collagen by a reverse transcription/polymerase chain reaction assay. All treatments significantly increased MMP-1 mRNA expression. The increase induced by chrysotile plus cigarette smoke was additive. Neither chrysotile, the RCFs, nor cigarette smoke alone affected MMP-2 mRNA expression. Combined exposure to chrysotile plus cigarette smoke or RCFs plus cigarette smoke significantly increased the level of MMP-2 mRNA expression. TIMP-1 mRNA expression was not detected in control, chrysotile or RCF exposed lungs. Cigarette smoke alone and cigarette smoke plus chrysotile induced TIMP-1 MRNA expression. TIMP-2 mRNA expression was not induced by either RCFs or chrysotile. Cigarette smoke significantly decreased TIMP-2 mRNA expression. This effect was not altered by RCF nor chrysotile coexposure. Neither RCFs nor chrysotile increased type-I collagen mRNA expression. Cigarette smoking decreased type-I collagen mRNA expression. Expression of type-IV collagen mRNA was not detected in any of the lung tissue samples. Foreign body giant cells and mononuclear cell infiltration around bronchioles were observed in lung tissues from chrysotile and chrysotile plus cigarette smoke exposed rats. No treatment related histopathological changes were seen in lung tissues from the other groups. The authors conclude that MMPs and TIMPs may be associated with remodeling of lung tissues induced by exposure to mineral fibers and cigarette smoke. |
| Doi | 10.1289/ehp.97105s51247 |
| Pmid | 9400732 |
| Wosid | WOS:A1997YH51000042 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | MMP; TIMP; asbestos; lung; ceramic fiber; chrysotile; collagen |