Determination of diaminotoluene isomers by gas chromatography
Brydia, LE; Willeboordse, FG
| HERO ID | 2068792 |
|---|---|
| Year | 1981 |
| Title | Determination of diaminotoluene isomers by gas chromatography |
| Book Title | Environmental carcinogens: Selected methods of analysis. Volume 4: Some aromatic amines and azo dyes in the general and industrial environment |
| Authors | Brydia, LE; Willeboordse, FG |
| Editor | Egan, H; Fishbein, L; O'Neill, IK; Castegnaro, M; Bartsch, H |
| Publisher Text | International Agency for Research on Cancer |
| City | Lyon, France |
| Page Numbers | 235-242 |
| Abstract | A method for determining isomers of diaminotoluene (25376458) was described. The method consisted of two procedures: procedure 1, which did not resolve the 2,4 and 2,5 isomers and procedure 2 which resolved all isomers. In procedure 1, 60 to 100 milligrams (mg) of sample were reacted with 1 milliliter (ml) trifluoroacetic-acid for 5 minutes, followed by reaction with 1ml trifluoroacetic-anhydride for 15 minutes. Ten microliters (microl) of sample was analyzed by a gas chromatograph (GC) using a column consisting of aluminum tubing packed with 2.0 percent neopentyl-glycol. In procedure 2, 60 to 100mg sample was shaken with chloroform, then reacted with trifluoroacetic-anhydride. The solution was then allowed to stand for 15 minutes. The solvent and excess reagent were evaporated in a stream of nitrogen. The sample was reconstituted by 1ml of acetone. A 5microl sample was injected into a GC equipped with a column consisting of aluminum tubing with 100 to 120 mesh Chromosorb. Repeated analyses of seven replicate samples of a synthetic sample according to procedure 1 produced a relative standard deviation of 0.7 to 8.4 percent. Analysis of 5 replicate samples of a crude product by procedure 2 had relative standard deviations of 0.6 to 7.7 percent for the 2,3, 3,4, 2,6, 2,4, and 2,5 isomers. The authors note that procedure 1 serves as an excellent method for quality control of factory grade material. Procedure 2 must be used when determining the 2,5 isomer is important. |
| Pmid | 7327675 |
| Wosid | WOS:A1981NH87600018 |
| Is Certified Translation | No |
| Dupe Override | No |
| Series | IARC Scientific Publications, no. 40 |
| Isbn | 9789283211402 |
| Is Public | Yes |
| Language Text | English |
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