Five new galactolipids from the freshwater microalga Porphyridium aerugineum and their nitric oxide inhibitory activity
Banskota, AH; Stefanova, R; Sperker, S; Melanson, R; Osborne, JA; O'Leary, SJB
| HERO ID | 2049785 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | Five new galactolipids from the freshwater microalga Porphyridium aerugineum and their nitric oxide inhibitory activity |
| Authors | Banskota, AH; Stefanova, R; Sperker, S; Melanson, R; Osborne, JA; O'Leary, SJB |
| Journal | Journal of Applied Phycology |
| Volume | 25 |
| Issue | 4 |
| Page Numbers | 951-960 |
| Abstract | Chemical investigation of the freshwater rhodophyte microalga Porphyridium aerugineum led to the isolation of five new galactolipids, namely, (2S)-1-O-eicosapentaenoyl-2-O-arachidonoyl-3-O-beta-d-galactopyranosylglycerol (1), (2S)-1-O-eicosapentaenoyl-2-O-linoleoyl-3-O-beta-d-galactopyranosylglycerol (2), (2S)-1-O-arachidoyl-2-O-palmitoyl-3-O-(beta-d-galactopyranosyl-6-1 alpha-d-galactopyranosyl)-glycerol (6), (2S)-1-O-eicosapentaenoyl-2-O-arachidoyl-3-O-(beta-d-galactopyranosyl-6-1 alpha-d-galactopyranosyl)-glycerol (7), and (2S)-1-O-eicosapentaenoyl-2-O-linoleoyl-3-O-(beta-d-galactopyranosyl-6-1 alpha-d-galactopyranosyl)-glycerol (8) together with five known galactolipids. The stereo-structures of all new galactolipids were elucidated by spectroscopic analyses and both enzymatic and chemical degradation methods. This is the first report of galactolipids from P. aerugineum. The newly isolated galactolipids showed strong and dose-dependent nitric oxide (NO) inhibitory activity against lipopolysaccharide-induced NO production in RAW264.7 macrophage cells. Both galactolipids 1 and 2 possessed stronger NO inhibitory activity than N (G)-methyl-l-arginine acetate salt, a well-known NO inhibitor used as a positive control. Further study suggested that these galactolipids inhibit NO production through downregulation of inducible nitric oxide synthase expression. |
| Doi | 10.1007/s10811-012-9935-9 |
| Wosid | WOS:000321588800004 |
| Url | http://link.springer.com/10.1007/s10811-012-9935-9 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Keyword | Porphyridium aerugineum; Rhodophyta; Galactolipid; Monogalactosyldiacylglycerol (MGDG) Digalactosyldiacylglycerol (DGDG); Nitric oxide; Inducible nitric oxide synthase |