Quantitative analysis of valiolamine through pre-column derivatization with phenylisocyanate using high-performance liquid chromatography with UV detection: Selection of reagent, identification of derivative and optimization of derivatization conditions
Li, He; Lee, J; Nguyen, D; Kim, S; Seo, Y; Chang, YK; Hong, SK; Moon, M; Chung, InY; Kim, CJ
| HERO ID | 2030140 |
|---|---|
| In Press | No |
| Year | 2009 |
| Title | Quantitative analysis of valiolamine through pre-column derivatization with phenylisocyanate using high-performance liquid chromatography with UV detection: Selection of reagent, identification of derivative and optimization of derivatization conditions |
| Authors | Li, He; Lee, J; Nguyen, D; Kim, S; Seo, Y; Chang, YK; Hong, SK; Moon, M; Chung, InY; Kim, CJ |
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume | 49 |
| Issue | 4 |
| Page Numbers | 957-963 |
| Abstract | This report describes the improved quantitative determination of valiolamine in a medium for microbial culture using high-performance liquid chromatography with UV detection. Valiolamine aqueous solution was dried, dissolved in dimethyl sulfoxide and derivatization performances of phenylisocyanate (PHI), 1-fluoro-2,4-dinitrobenznene and 1-naphthylisothiocyanate were compared in the presence of triethylamine. The PHI was chosen as the most suitable derivatization reagent and the valiolamine-PHI derivative was identified by thin-layer chromatography and electrospray ionization mass spectrometry. The derivative eluted at 10.5 min on a reverse-phase column using a mobile phase composed of 10% acetonitrile in water containing 0.5 mM sodium octyl sulfate (pH 3.0), at a column flow rate of 1.0 mL/min with UV detection at 240 nm. The optimum conditions for derivatization were a reaction temperature of 30 degrees C, reaction time of 30 min, and PHI concentration higher than 33.6 mM. Calibration curves were linear in the range of 0.99-19.95 microg/mL for the standard solutions and 24.9-99.7 microg/mL for the spiked sample. The proposed method was validated and proven to be selective, accurate and precise and suitable for the quantitative analysis of valiolamine in medium for microbial cultures. |
| Doi | 10.1016/j.jpba.2009.02.020 |
| Pmid | 19303235 |
| Wosid | WOS:000265046200013 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-62949164447&doi=10.1016%2fj.jpba.2009.02.020&partnerID=40&md5=b28e4d94c3089557528a2f23cec317d0 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Valiolamine; Phenylisocyanate; Derivatization; HPLC; UV detection |