Rat liver foci and in vitro assays to detect initiating and promoting effects of chlorinated ethanes and ethylenes
Milman, HA; Story, DL; Riccio, ES; Sivak, A; Tu, AS; Williams, GM; Tong, C; Tyson, CA
HERO ID
200479
Reference Type
Journal Article
Year
1988
Language
English
PMID
| HERO ID | 200479 |
|---|---|
| In Press | No |
| Year | 1988 |
| Title | Rat liver foci and in vitro assays to detect initiating and promoting effects of chlorinated ethanes and ethylenes |
| Authors | Milman, HA; Story, DL; Riccio, ES; Sivak, A; Tu, AS; Williams, GM; Tong, C; Tyson, CA |
| Journal | Annals of the New York Academy of Sciences |
| Volume | 534 |
| Page Numbers | 521-530 |
| Abstract | Nine chlorinated aliphatics (CAs) were examined in a rat liver foci assay for tumor initiating and promoting activities. In this model, young adult male Osborne Mendel rats were first subjected to a partial hepatectomy, the test chemical was then administered at the maximum tolerated dose in the initiation or promotion phase in conjunction with diethylnitrosamine (DEN; 30 mg/kg b.w.) or phenobarbital (PB; 0.05 percent, w/w, in the diet), and gamma glutamyltranspeptidase (GGT) was used as a putative preneoplastic indicator. When administered in the promotion protocol after initiation with DEN, 1,1-dichloroethane, 1,1,2-trichloroethane (1,1,2-TCE), 1,1,2,2-tetrachloroethane (1,1,2,2-TTCE), tetrachloroethylene (TTCY), and hexachloroethane induced significant increases in GGT+-foci above control levels. 1,1,2,2-TTCE, TTCY, and 1,1,2-TCE also induced significant increases in GGT+-foci when administered in the promotion protocol without DEN initiation. Two variants of GGT+-foci were observed: the classical type associated with PB promotion, and the other, which was more diffuse, less intensely stained, resembling foci undergoing redifferentiation and associated with CAs. A number of CAs were also genotoxic in short-term in vitro tests. Taken together, the studies suggest that CAs may be complete carcinogens in vivo with weak initiating activity and stronger promoting activity |
| Doi | 10.1111/j.1749-6632.1988.tb30143.x |
| Pmid | 3389679 |
| Wosid | WOS:A1988Q080100049 |
| Url | https://search.proquest.com/docview/78300109?accountid=171501 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Adult; analogs & derivatives; Animals; Body Weight; Carcinogens; Cell Transformation,Neoplastic; Diet; Diethylnitrosamine; drug effects; Ethane; Ethylene Dichlorides; Ethylenes; Hepatectomy; Hydrocarbons; Hydrocarbons,Chlorinated; In Vitro; Liver; Male; Mice; Mutagenicity Tests; Organ Size; Pesticides; Phenobarbital; Rats; Research Support,U.S.Gov't,Non-P.H.S.; Tetrachloroethylene; toxicity; Trichloroethanes |
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