A procedure for the quantitative measurement of the mutagenicity of volatile liquids in the Ames Salmonella/microsome assay
Barber, ED; Donish, WH; Mueller, KR
HERO ID
200219
Reference Type
Journal Article
Year
1981
Language
English
PMID
| HERO ID | 200219 |
|---|---|
| In Press | No |
| Year | 1981 |
| Title | A procedure for the quantitative measurement of the mutagenicity of volatile liquids in the Ames Salmonella/microsome assay |
| Authors | Barber, ED; Donish, WH; Mueller, KR |
| Journal | Mutation Research: Genetic Toxicology |
| Volume | 90 |
| Issue | 1 |
| Page Numbers | 31-48 |
| Abstract | We have designed a closed, inert incubation system for testing the mutagenicity of volatile compounds. The containment properties of this system have been investigated using carbon-14 labelled 1,2-dibromoethane. The recovery of this solvent was about 95% following a 48-h incubation at 37 degrees. Using the Ames Salmonella/microsome mutagenicity assay we have determined the mutagenic potency of 10 common halogenated alkane solvents. Of these 10 compounds, only 1,2-dibromoethane and 1-bromo-2-chloroethane give positive results in the standard test procedure, whereas 7 of the 10 give positive results in the closed system. The specificity observed for reversion of the tester strains and the lack of any significant effect of added rat-liver "S9" fractions suggest that these haloalkanes are direct-acting "base-pair" type mutagens. The mutagenic potencies of the 7 positive compounds range from 0.001 revertants per nanomole for 1,2-dichloroethane to 0.172 revertants per nanomole for 1,2-dibromoethane. A minimum or threshold response level for each material has been calculated |
| Doi | 10.1016/0165-1218(81)90048-3 |
| Pmid | 7029269 |
| Wosid | WOS:A1981MD78600004 |
| Url | http://www.sciencedirect.com/science/article/pii/0165121881900483 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: MUTAT RES 90 ISSN: |
| Is Public | Yes |
| Language Text | English |
| Keyword | Ethylene Dibromide; genetics; Hydrocarbons,Halogenated; instrumentation; methods; Mutagenicity Tests; Mutagens; pharmacology; Salmonella typhimurium; Volatilization |
| Relationship(s) |
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