Microscopic detection of yeasts using fluorescence in situ hybridization
Inácio, J; da Luz Martins, M
| HERO ID | 1998415 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | Microscopic detection of yeasts using fluorescence in situ hybridization |
| Authors | Inácio, J; da Luz Martins, M |
| Journal | Methods in Molecular Biology |
| Volume | 968 |
| Page Numbers | 71-82 |
| Abstract | Fluorescence in situ hybridization (FISH) has been widely used for the detection and identification of microorganisms in their natural environments. In this chapter we describe the use of a simple FISH-based protocol to detect and identify clinically relevant yeast species in culture and biological samples using Cryptococcus neoformans as a model. After fixation of cells with paraformaldehyde, the same are embedded in hybridization buffer containing specific fluorochrome-labeled oligonucleotide probes. After incubation and a subsequent washing step for removing unbound probes, samples are analyzed by epifluorescence microscopy. |
| Doi | 10.1007/978-1-62703-257-5_5 |
| Pmid | 23296886 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |