The cellular compatibility of five endodontic sealers during the setting period
Schwarze, T; Fiedler, I; Leyhausen, G; Geurtsen, W
| HERO ID | 1983050 |
|---|---|
| In Press | No |
| Year | 2002 |
| Title | The cellular compatibility of five endodontic sealers during the setting period |
| Authors | Schwarze, T; Fiedler, I; Leyhausen, G; Geurtsen, W |
| Journal | Journal of Endodontics |
| Volume | 28 |
| Issue | 11 |
| Page Numbers | 784-786 |
| Abstract | The purpose of this study was to evaluate the cellular compatibility of five endodontic sealers in the first 24 h after mixing. Specimens of N2, Endomethasone, Apexit, AH Plus, and Ketac Endo were extracted with cell culture medium 0, 1, 5, and 24 h after mixing. Eluates were tested for cytotoxicity with immortal 3T3 cells and primary human periodontal ligament fibroblasts using XTT-assays. Data were analyzed for statistically significant differences by means of Dunnett's t tests (p < 0.05). All extracts of N2 completely inhibited cell metabolism (p < 0.05). Similar effects were provoked by the first three eluates of Endomethasone, but the 24-h extract irritated cells significantly less (p < 0.05). Severe cytotoxicity was also observed with all Ketac Endo extracts (p < 0.05). A significant inhibition of mitochondrial activity was induced by the first (3T3) or the first and second eluate (periodontal ligament fibroblasts) of AH Plus (p < 0.05). The subsequent eluates of this sealer and all extracts of Apexit did not reveal any cytotoxic potency. |
| Doi | 10.1097/00004770-200211000-00009 |
| Pmid | 12470025 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | 3T3 Cells; Administration, Topical; Animals; Anti-Inflammatory Agents/toxicity; Biocompatible Materials/chemistry/ toxicity; Calcium Hydroxide/toxicity; Dexamethasone/toxicity; Drug Combinations; Epoxy Resins/toxicity; Eugenol/toxicity; Fibroblasts/drug effects; Formaldehyde/toxicity; Glass Ionomer Cements/toxicity; Hydrocortisone; Indicators and Reagents; Irritants/toxicity; Periodontal Ligament/cytology/drug effects; Root Canal Filling Materials/chemistry/ toxicity; Statistics as Topic; Tetrazolium Salts/diagnostic use; Thymol/ analogs & derivatives/toxicity; Time Factors; Zinc Oxide/toxicity |