Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for gram-negative bacteria

Sondi, I; Salopek-Sondi, B

HERO ID

196277

Reference Type

Journal Article

Year

2004

Language

English

PMID

15158396

HERO ID 196277
In Press No
Year 2004
Title Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for gram-negative bacteria
Authors Sondi, I; Salopek-Sondi, B
Journal Journal of Colloid and Interface Science
Volume 275
Issue 1
Page Numbers 177-182
Abstract The antimicrobial activity of silver nanoparticles against E. coli was investigated as a model for Gram-negative bacteria. Bacteriological tests were performed in Luria?Bertani (LB) medium on solid agar plates and in liquid systems supplemented with different concentrations of nanosized silver particles. These particles were shown to be an effective bactericide. Scanning and transmission electron microscopy (SEM and TEM) were used to study the biocidal action of this nanoscale material. The results confirmed that the treated E. coli cells were damaged, showing formation of ?pits? in the cell wall of the bacteria, while the silver nanoparticles were found to accumulate in the bacterial membrane. A membrane with such a morphology exhibits a significant increase in permeability, resulting in death of the cell. These nontoxic nanomaterials, which can be prepared in a simple and cost-effective manner, may be suitable for the formulation of new types of bactericidal materials.
Doi 10.1016/j.jcis.2004.02.012
Pmid 15158396
Is Certified Translation No
Dupe Override No
Comments N1-Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteriaID-14
Is Public Yes
Language Text English
Keyword Bacteria; Bactericide; E. coli; Nanoparticles; Silver
Is Qa No