Proteomic analysis of the mode of antibacterial action of silver nanoparticles
Lok, CN; Ho, CM; Chen, R; He, QY; Yu, WY; Sun, HZ; Tam, PKH; Chiu, JF
| HERO ID | 196268 |
|---|---|
| In Press | No |
| Year | 2006 |
| Title | Proteomic analysis of the mode of antibacterial action of silver nanoparticles |
| Authors | Lok, CN; Ho, CM; Chen, R; He, QY; Yu, WY; Sun, HZ; Tam, PKH; Chiu, JF |
| Journal | Journal of Proteome Research |
| Volume | 5 |
| Issue | 4 |
| Page Numbers | 916-924 |
| Abstract | Silver nanoparticles (nano-Ag) are potent and broad-spectrum antimicrobial agents. In this study, spherical nano-Ag (average diameter = 9.3 nm) particles were synthesized using a borohydride reduction method and the mode of their antibacterial action against E. coli was investigated by proteomic approaches (2-DE and MS identification), conducted in parallel to analyses involving solutions of Ag(+) ions. The proteomic data revealed that a short exposure of E. coli cells to antibacterial concentrations of nano-Ag resulted in an accumulation of envelope protein precursors, indicative of the dissipation of proton motive force. Consistent with these proteomic findings, nano-Ag were shown to destabilize the outer membrane, collapse the plasma membrane potential and deplete the levels of intracellular ATP. The mode of action of nano-Ag was also found to be similar to that of Ag(+) ions (e.g., Dibrov, P. et al, Antimicrob. Agents Chemother. 2002, 46, 2668-2670); however, the effective concentrations of nano-Ag and Ag(+) ions were at nanomolar and micromolar levels, respectively. Nano-Ag appear to be an efficient physicochemical system conferring antimicrobial silver activities. |
| Doi | 10.1021/pr0504079 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | N1-Proteomic analysis of the mode of antibacterial action of silver nanoparticlesID-10 |
| Is Public | Yes |
| Is Qa | No |