Determination of glutathione in plant extracts as the 1-chloro-2,4-dinitrobenzene conjugate in the presence of glutathione S-transferase
Hermsen, W; Mcmahon, PJ; Anderson, JW
| HERO ID | 1960870 |
|---|---|
| In Press | No |
| Year | 1997 |
| Title | Determination of glutathione in plant extracts as the 1-chloro-2,4-dinitrobenzene conjugate in the presence of glutathione S-transferase |
| Authors | Hermsen, W; Mcmahon, PJ; Anderson, JW |
| Journal | Plant Physiology and Biochemistry |
| Volume | 35 |
| Issue | 6 |
| Page Numbers | 491-496 |
| Abstract | A spectrophotometric method is described for the detection of total glutathione (oxidized and reduced) in plant extracts based on the conjugation of reduced glutathione with 1-chloro-2,4-dinitrobenzene (CDNB) in the presence of dithioerythritol and glutathione S-transferase (EC 2.5.1.18) at pH 6.5. Glutathione S-transferase also supported a reaction with homoglutathione at about 5% of the rate with glutathione but, at equilibrium, the absorbance change for the two substrates was similar. The glutathione content of acidic extracts of wheat (Triticum aestivum var. Condor) roots as determined by the 1-chloro-2,4-dinitro-benzene (CDNB) method was 6.5% less than that obtained by the HPLC method based on the fluorescence of the monobromobimane derivative. The recovery of exogenous glutathione, added to the extracts, exceeded 90%. The method is suited to the specific determination of glutathione in poaecean species which also contain hydroxymethyl-glutathione since glutathione S-transferase reportedly does not support a reaction with this compound. |
| Wosid | WOS:A1997XF72400009 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030972068&partnerID=40&md5=df9936ceb9b56c9fe1eda26f7c6d8a8e |
| Is Public | Yes |
| Language Text | English |
| Keyword | 1-chloro-2,4-dinitrobenzene; glutathione; glutathione determination; glutathione S-transferase; Triticum aestivum |