Comparison of acute responses of mice livers to short-term exposure to nano-sized or micro-sized silver particles

Cha, K; Hong, H; Choi, Y; Lee, MJ; Park, JH; Chae, H; Ryu, G; Myung, H

HERO ID

195498

Reference Type

Journal Article

Year

2008

Language

English

PMID

18604478

HERO ID 195498
In Press No
Year 2008
Title Comparison of acute responses of mice livers to short-term exposure to nano-sized or micro-sized silver particles
Authors Cha, K; Hong, H; Choi, Y; Lee, MJ; Park, JH; Chae, H; Ryu, G; Myung, H
Journal Biotechnology Letters
Volume 30
Issue 11
Page Numbers 1893-1899
Abstract Mice were fed either 13 nm silver nanoparticles or 2–3.5 μm silver microparticles. The livers were then obtained after 3 days and subjected to a histopathological analysis. The nanoparticle-fed and microparticle-fed livers both exhibited lymphocyte infiltration in the histopathological analysis, suggesting the induction of inflammation. In vitro, a human hepatoma cell line (Huh-7) was treated with the same silver nanoparticles and microparticles. The mitochondrial activity and glutathione production were hardly affected. However, the DNA contents decreased 15% in the nanoparticle-treated cells and 10% in the microparticle-treated cell, suggesting a more potent induction of apoptosis by the nanoparticles. From a microarray analysis of the RNA from the livers of the nano- and micro-particle-fed mice, the expression of genes related to apoptosis and inflammation was found to be altered. These gene expression changes in the nanoparticle-treated livers lead to phenotypical changes, reflecting increased apoptosis and inflammation. The changes in the gene expression were confirmed by using a semi-quantitative RT-PCR.
Doi 10.1007/s10529-008-9786-2
Pmid 18604478
Is Certified Translation No
Dupe Override No
Comments N1-Comparison of acute responses of mice livers to short-term exposure to nano-sized or micro-sized silver particlesID-37
Is Public Yes
Language Text English
Is Qa No
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