[Extract human DNA from maggot crop contents by phenol-chloroform method coupled with paramagnetic particle method]
Wang, X; Cai, JF; Zhong, M; Li, JB; Zhang, JG; Lan, LM
| HERO ID | 1940786 |
|---|---|
| In Press | No |
| Year | 2009 |
| Title | [Extract human DNA from maggot crop contents by phenol-chloroform method coupled with paramagnetic particle method] |
| Authors | Wang, X; Cai, JF; Zhong, M; Li, JB; Zhang, JG; Lan, LM |
| Journal | Fayixue Zazhi |
| Volume | 25 |
| Issue | 6 |
| Page Numbers | 421-424 |
| Abstract | <strong>OBJECTIVE: </strong>To establish an effective phenol-chloroform method coupled with paramagnetic particle method for human DNA extraction from maggot crop contents in STR genotyping.<br /><br /><strong>METHODS: </strong>Human DNA was extracted from the maggot crop contents using phenol-chloroform method and purified by paramagnetic particle method. DNA was quantified by PCR with Quantifiler Human DNA Quantification Kit using 7500 real-time fluorescence quantitative PCR instrument. PCR products were genotyped by AmpFlSTR Identifiler PCR Amplification Kit using 3130XL-Avant genetic analyzer.<br /><br /><strong>RESULTS: </strong>The template DNA yield by the method described were increased at least 2 times than the phenol-chloroform extraction method alone. All of the full 16 STR profiles could be obtained with the samples extracted by this method when the DNA yield reached (0.218 +/- 0.041) ng/microL.<br /><br /><strong>CONCLUSION: </strong>Phenol-chloroform method coupled with paramagnetic particle method can effectively increase the sensitivity of STR analysis of human DNA recovered from maggot crop contents and is a valuable tool for forensic entomology. |
| Pmid | 20225616 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | Chinese |