Development of an improved method to extract pesticide residues in foods using acetonitrile with magnesium sulfate and chloroform

Liu, G; Rong, L; Guo, B; Zhang, M; Li, S; Wu, Q; Chen, J; Chen, B; Yao, S

HERO ID

1940569

Reference Type

Journal Article

Year

2011

Language

English

PMID

21296358

HERO ID 1940569
In Press No
Year 2011
Title Development of an improved method to extract pesticide residues in foods using acetonitrile with magnesium sulfate and chloroform
Authors Liu, G; Rong, L; Guo, B; Zhang, M; Li, S; Wu, Q; Chen, J; Chen, B; Yao, S
Journal Journal of Chromatography A
Volume 1218
Issue 11
Page Numbers 1429-1436
Abstract A multiresidue method was developed based on extraction of 10 g sample with 10 mL acetonitrile and subsequent liquid-liquid partitioning formed by adding 4 g MgSO₄ plus 1 mL chloroform. During the partitioning process, the extraction recoveries of polar analytes were found to be essentially determined by the acetonitrile content in the aqueous phase. The use of MgSO₄ gave the least acetonitrile left in the aqueous phase (lower than 5%) and thus promoting complete partitioning of analytes into the organic phase. At the same time, removal of water from the acetonitrile phase was achieved by adding only a small amount of chloroform with no influence on the acetonitrile content in the aqueous phase, thus leading to decreasing the co-extraction of polar matrix components. The most complete mutual separation of acetonitrile and water was achieved by the joint use of MgSO₄ and chloroform and thus the optimal extraction recovery and analytical selectivity were obtained simultaneously. The new method, with higher recoveries of polar analytes, better analytical selectivity and simpler manipulation, is a claimed improvement to the original QuEChERS method. The proposed method was finally validated by the determination of 20 pesticides in a mixed food matrix by using liquid chromatography tandem mass spectrum (LC-MS/MS). Acceptable linearity, sensitivity, recovery, precision and selectivity results were obtained.
Doi 10.1016/j.chroma.2011.01.041
Pmid 21296358
Wosid WOS:000288305200002
Is Certified Translation No
Dupe Override No
Is Public Yes
Language Text English
Keyword Multiresidue method; QuEChERS method; LC-MS/MS; Acetonitrile; MgSO4; Chloroform