Pulmonary and systemic distribution of inhaled ultrafine silver particles in rats

Takenaka, S; Karg, E; Roth, C; Schulz, H; Ziesenis, A; Heinzmann, U; Schramel, P; Heyder, J

HERO ID

19055

Reference Type

Journal Article

Year

2001

Language

English

PMID

11544161

HERO ID 19055
In Press No
Year 2001
Title Pulmonary and systemic distribution of inhaled ultrafine silver particles in rats
Authors Takenaka, S; Karg, E; Roth, C; Schulz, H; Ziesenis, A; Heinzmann, U; Schramel, P; Heyder, J
Journal Environmental Health Perspectives
Volume 109
Issue Suppl. 4
Page Numbers 547-551
Abstract The cardiovascular system is currently considered a target for particulate matter, especially for ultrafine particles. In addition to autonomic or cytokine mediated effects, the direct interaction of inhaled materials with the target tissue must be examined to understand the underlying mechanisms. In the first approach, pulmonary and systemic distribution of inhaled ultrafine elemental silver (EAg) particles was investigated on the basis of morphology and inductively coupled plasma mass spectrometry (ICP-MS) analysis. Rats were exposed for 6 hr at a concentration of 133 Ág EAg m3 (3 * 106 cm3, 15 nm modal diameter) and were sacrificed on days 0, 1, 4, and 7. ICP-MS analysis showed that 1.7 Ág Ag was found in the lungs immediately after the end of exposure. Amounts of Ag in the lungs decreased rapidly with time, and by day 7 only 4% of the initial burden remained. In the blood, significant amounts of Ag were detected on day 0 and thereafter decreased rapidly. In the liver, kidney, spleen, brain, and heart, low concentrations of Ag were observed. Nasal cavities, especially the posterior portion, and lung-associated lymph nodes showed relatively high concentrations of Ag. For comparison, rats received by intratracheal instillation either 150 ÁL aqueous solution of 7 Ág silver nitrate (AgNO3) (4.4 Ág Ag) or 150 ÁL aqueous suspension of 50 Ág agglomerated ultrafine EAg particles. A portion of the agglomerates remained undissolved in the alveolar macrophages and in the septum for at least 7 days. In contrast, rapid clearance of instilled water-soluble AgNO3 from the lung was observed. These findings show that although instilled agglomerates of ultrafine EAg particles were retained in the lung, Ag was rapidly cleared from the lung after inhalation of ultrafine EAg particles, as well as after instillation of AgNO3, and entered systemic pathways.
Doi 10.2307/3454667
Pmid 11544161
Wosid WOS:000170700500012
Url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1240579/?tool=pubmed
Is Certified Translation No
Dupe Override No
Comments ECRIB.Environ. Health Perspect. 109(suppl. 4): 547-551.
Is Public Yes
Language Text English
Keyword distribution; ICP-MS; inhalation; instillation; morphology; silver; ultrafine
Is Qa No