Heme Oxygenase Activity and Cytochrome P-450 Content Associated with Induced Metallothionein in the Liver of Rats Treated with Various Metals
Arizono, K; Okanari, E; Ueno, K; Ariyoshi, T
| HERO ID | 1875459 |
|---|---|
| Year | 1991 |
| Title | Heme Oxygenase Activity and Cytochrome P-450 Content Associated with Induced Metallothionein in the Liver of Rats Treated with Various Metals |
| Authors | Arizono, K; Okanari, E; Ueno, K; Ariyoshi, T |
| Volume | A26 |
| Page Numbers | Environmental Science and Engineering |
| Abstract | The relationship between heme oxygenase (HO) activity and cytochrome-p-450 content associated with induced metallothionein (MT) was investigated in the liver of rats treated with various metals. Adult male Wistar-rats were injected intraperitoneally with cadmium-chloride (10108642), 3mg/kg; cobalt-chloride (7646799), 20mg/kg; zinc-acetate (557346), 33mg/kg; nickel-chloride (7718549), 40mg/kg; copper-sulfate (7758987), 15mg/kg; or manganous-acetate (638380), 45mg/kg and killed 24 or 72 hours later. Control rats were injected with 0.9% saline alone at the corresponding volumes per kilogram. The responses to treatments with metals of the A-series (cadmium, zinc, copper) that could bind to MT in-vivo and metals of the B-series (cobalt, nickel, manganese) that could not bind to MT in-vivo were compared. A single treatment with any metal in the A-series or B-series increased both hepatic HO activity and MT concentration. Repeated treatments with metals of the A-series, however, decreased HO activities more than a single injection despite appreciable increases in MT concentrations. Repeated treatments with metals of the B-series increased both HO activities and MT concentrations. Cytochrome-P-450 concentrations were depressed by single or repeated treatments with all metals tested. The authors conclude that the magnitude of hepatic HO activity is regulated by both the amount of zinc in the zinc-MT complex induced by the metal and by the ability of the metal to bind thionein. Additionally, some metals affect cytochrome-P-450 heme degradation regardless of the magnitude of HO activity. |
| Doi | 10.1080/10934529109375676 |
| Wosid | WOS:A1991FV16300007 |
| Report Number | NIOSH/00202056 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: Journal of Environmental Science and Health ISSN: |
| Is Public | Yes |
| Language Text | English |