Human serum albumin coordinates Cu(II) at its N-terminal binding site with 1 pM affinity
Rozga, M; Sokolowska, M; Protas, AM; Bal, W
HERO ID
1848672
Reference Type
Journal Article
Year
2007
Language
English
PMID
| HERO ID | 1848672 |
|---|---|
| In Press | No |
| Year | 2007 |
| Title | Human serum albumin coordinates Cu(II) at its N-terminal binding site with 1 pM affinity |
| Authors | Rozga, M; Sokolowska, M; Protas, AM; Bal, W |
| Journal | Journal of Biological Inorganic Chemistry |
| Volume | 12 |
| Issue | 6 |
| Page Numbers | 913-918 |
| Abstract | The conditional stability constant at pH 7.4 for Cu(II) binding at the N-terminal site (NTS) of human serum albumin (HSA) was determined directly by competitive UV-vis spectroscopy titrations using nitrilotriacetic acid (NTA) as the competitor in 100 mM NaCl and 100 mM N-(2-hydroxyethyl)piperazine-N'-ethanesulfonic acid (Hepes). The log Kc (NTS) value of 12.0 +/- 0.1 was determined for HSA dissolved in 100 mM NaCl. A false log log Kc (NTS) (c) value of 11.4 +/- 0.1 was obtained in the 100 mM Hepes buffer, owing to the formation of a ternary Cu(NTA)(Hepes) complex. The impact of the picomolar affinity of HSA for Cu(II) on the availability of these ions in neurodegenerative disorders is briefly discussed. |
| Doi | 10.1007/s00775-007-0244-8 |
| Pmid | 17516096 |
| Wosid | WOS:000248414100018 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | human serum albumin; copper(II); conditional stability constant |