Induction of micronuclei in Syrian hamster embryo cells: Comparison to results in the SHE cell transformation assay for National Toxicology Program test chemicals

Gibson, DP; Brauninger, R; Shaffi, HS; Kerckaert, GA; LeBoeuf, RA; Isfort, RJ; Aardema, MJ

HERO ID

184374

Reference Type

Journal Article

Year

1997

Language

English

PMID

9269331

HERO ID 184374
In Press No
Year 1997
Title Induction of micronuclei in Syrian hamster embryo cells: Comparison to results in the SHE cell transformation assay for National Toxicology Program test chemicals
Authors Gibson, DP; Brauninger, R; Shaffi, HS; Kerckaert, GA; LeBoeuf, RA; Isfort, RJ; Aardema, MJ
Journal Mutation Research: Genetic Toxicology and Environmental Mutagenesis
Volume 392
Issue 1-2
Page Numbers 61-70
Abstract Sixteen chemicals currently being tested in National Toxicology Program (NTP) carcinogenicity studies were evaluated in the Syrian hamster embryo (SHE) cell in vitro micronucleus assay. Results from these studies were compared to the results from the SHE cell transformation assay for the same chemicals The overall concordance between induction of micronuclei and transformation of SHE cells was 56%, which is far lower that the 93% concordance between these two tests reported previously by Fritzenschaf et al. (1993; Mutation Res. 319, 47-53). The difference between our results appears to be due to differences in the types of chemicals in the two studies. Overall, there is good agreement between the SHE cell micronucleus and transformation assays for mutagenic chemicals, but, as our study highlights, the SHE cell transformation assay has the added utility of detecting nonmutagenic carcinogens. The utility of a multi-endpoint assessment in SHE cells for carcinogen screening is discussed.
Doi 10.1016/s0165-1218(97)00045-1
Pmid 9269331
Wosid WOS:A1997XQ83400007
Is Certified Translation No
Dupe Override No
Is Public Yes
Language Text English
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