X-ray structure of the metal-sensor CnrX in both the apo- and copper-bound forms
Pompidor, G; Maillard, AP; Girard, E; Gambarelli, S; Kahn, R; Coves, J
| HERO ID | 1840490 |
|---|---|
| In Press | No |
| Year | 2008 |
| Title | X-ray structure of the metal-sensor CnrX in both the apo- and copper-bound forms |
| Authors | Pompidor, G; Maillard, AP; Girard, E; Gambarelli, S; Kahn, R; Coves, J |
| Journal | FEBS Letters |
| Volume | 582 |
| Issue | 28 |
| Page Numbers | 3954-3958 |
| Abstract | Both the X-ray structures of the apo- and the copper-bound forms of the metal-sensor domain (residues 31-148) of CnrX from Cupriavidus metallidurans CH34 were obtained at 1.74A resolution from a selenomethionine derivative. This four-helix hooked-hairpin is the first structure of a metal-sensor in an ECF-type signaling pathway. The copper ion is bound in a type 2-like center with a 3N1O coordination in the equatorial plane and shows an unprecedented remote fifth axial ligand with Met93 contributing a weak S-Cu bond. The signal onset cannot be explained by conformational changes associated with CnrX metallation. |
| Doi | 10.1016/j.febslet.2008.10.042 |
| Pmid | 18992246 |
| Wosid | WOS:000261235000020 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000261235000020 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Metal-sensor; Heavy-metal; Signal transduction; X-ray structure; CnrX; Cupriavidus metallidurans CH34 |