Improved ex vivo/in vitro lymph node cell proliferation assay in guinea pigs for a screening test of contact hypersensitivity of chemical compounds
Kashima, R; Oyake, Y; Okada, J; Ikeda, Y
| HERO ID | 1808670 |
|---|---|
| In Press | No |
| Year | 1996 |
| Title | Improved ex vivo/in vitro lymph node cell proliferation assay in guinea pigs for a screening test of contact hypersensitivity of chemical compounds |
| Authors | Kashima, R; Oyake, Y; Okada, J; Ikeda, Y |
| Journal | Toxicology |
| Volume | 114 |
| Issue | 1 |
| Page Numbers | 47-55 |
| Abstract | BIOSIS COPYRIGHT: BIOL ABS. Simple and efficient ex vivo/in vitro screening systems for contact allergens are developed for alternative to conventional animal tests. We have previously proposed an ex vivo/in vitro proliferation assay as a first stage screening method with advantages over existing alternatives, using lymph node cells (LNC) from sensitized guinea pigs of the Hartley strain. In this study, we have first confirmed, by histochemical analysis using in vivo bromodeoxyuridine (BrdU) and pyronin staining, that the ex vivo/in vitro LNC proliferation reflects in vivo response of lymph nodes to contact allergens. Furthermore, to improve the LNC assay, we then have investigated several experimental conditions for their influences on the LNC assay, demonstrating that, (1) the subscapular and the cervical LNC responded highly to contact allergens, (2) among three cervical lymph nodes the superficial dorsal cervical lymph nodes were the most reactive, (3) several vehicles alone used for animal se |
| Doi | 10.1016/S0300-483X(96)89846-5 |
| Pmid | 8931760 |
| Wosid | WOS:A1996VT72100006 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030589079&doi=10.1016%2fS0300-483X%2896%2989846-5&partnerID=40&md5=8be6b51a9b9c337f7f889664f50688a1 |
| Is Public | Yes |
| Language Text | English |
| Keyword | <?xml version="1.0" encoding="UTF-8"?><kw>Biochemical Studies-General</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Biophysics-General Biophysical Techniques</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Pathology</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Toxicology-General</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Immunology and Immunochemistry-Immunopathology</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Allergy</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Caviidae</kw> |