Optimization of the mouse ear swelling test for in vivo and in vitro studies of weak contact sensitizers
Garrigue J-L; Nicholas J-F; Fraginals, R; Benezra, C; Bour, H; Schmitt, D
| HERO ID | 1808615 |
|---|---|
| In Press | No |
| Year | 1994 |
| Title | Optimization of the mouse ear swelling test for in vivo and in vitro studies of weak contact sensitizers |
| Authors | Garrigue J-L; Nicholas J-F; Fraginals, R; Benezra, C; Bour, H; Schmitt, D |
| Journal | Contact Dermatitis |
| Volume | 30 |
| Issue | 4 |
| Page Numbers | 231-237 |
| Abstract | BIOSIS COPYRIGHT: BIOL ABS. Murine models for assessment of the contract sensitizing properties of chemicals rely on mouse ear swelling tests (Mest), which are not sensitive enough to detect weak sensitizers. The aim of the present study was to develop in mice and adjuvant-free Mest appropriate for in vivo detection of any type of sensitizer (weak to strong), and useful for in vitro assessment of contact sensitivity (CS). 3 haptens were tested: dinitrochlorobenzene (DCNB), para-phenylenediamine (pPD) and isoeugenol. We compared various protocols for induction of the CS reaction, differing by the site of induction, the number of applications and the concentrations of the 3 haptens. Comparison of the induction site for optimal Cs reaction showed that, in Balb/c mice, the back was a better site of induction than the abdomen. Detection of the sensitizing properties of weak sensitizers (pPD, isoeugenol) was possible using an adjuvant-free protocol, provided that the induction phase comprised hapten app |
| Doi | 10.1111/j.1600-0536.1994.tb00650.x |
| Pmid | 8033550 |
| Wosid | WOS:A1994NJ12400009 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:A1994NJ12400009Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028347374&doi=10.1111%2fj.1600-0536.1994.tb00650.x&partnerID=40&md5=48c8710ef668fbeeca5c6b9bb4bb39c1 |
| Is Public | Yes |
| Language Text | English |
| Keyword | <?xml version="1.0" encoding="UTF-8"?><kw>Cytology and Cytochemistry-Animal</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Biochemical Methods-General</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Biochemical Studies-General</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Pathology</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Metabolism-General Metabolism</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Blood</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Integumentary System-General</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Integumentary System-Anatomy</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Integumentary System-Pathology</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Toxicology-General</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>In Vitro Studies</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Immunology and Immunochemistry-Immunopathology</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Allergy</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Public Health: Environmental Health-Occupational Health</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Muridae</kw> |
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