Combined effects of nitric oxide and hyperoxia on surfactant function and pulmonary inflammation
Robbins, CG; Davis, JM; Merritt, TA; Amirkhanian, JD; Sahgal, N; Morin, FC; Horowitz, S
| HERO ID | 1663935 |
|---|---|
| In Press | No |
| Year | 1995 |
| Title | Combined effects of nitric oxide and hyperoxia on surfactant function and pulmonary inflammation |
| Authors | Robbins, CG; Davis, JM; Merritt, TA; Amirkhanian, JD; Sahgal, N; Morin, FC; Horowitz, S |
| Journal | American Journal of Physiology |
| Volume | 269 |
| Issue | 4 Pt 1 |
| Page Numbers | L545-L550 |
| Abstract | NO and its derivative ONOO- are potent free radicals that can cause cell damage, especially in the presence of O2. To determine the potential pulmonary toxicities of nitric oxide (NO) and peroxynitrite (ONOO-) in vitro, Survanta (2.5 mg/ml) was exposed to ONOO- (0.3-8 mM) in the presence of two different buffering systems (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid and phosphate buffer) and minimum surface tension (MST) was determined with an oscillating bubble surfactometer. Significant increases in MST were seen only with exposure to 8 mM ONOO-, indicating that in vitro, high concentrations of ONOO- can inhibit natural surfactant function. The in vivo effects of NO and hyperoxia were then studied in four groups of newborn piglets ventilated for 48 h with 21% O2, 100% O2, 21% O2 and 100 ppm NO, or with 90% O2 and 100 ppm NO. Five animals served as an untreated control group. Bronchoalveolar lavage fluid (BAL) obtained at 48 h was subjected to centrifugation and the surfactant pellet was reconstituted to 5 mg phospholipid/ml. Significant increases in MST were seen in surfactant from piglets ventilated with NO and 90% O2, compared with either untreated controls or piglets ventilated with 21% O2 for 48 h (P < 0.05, analysis of variance). Significant increases in neutrophil chemotactic activity (NCA) of BAL were also found in the NO and O2 group (P < 0.05), with significant positive interaction between NO and O2 found (P < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS) |
| Doi | 10.1152/ajplung.1995.269.4.L545 |
| Pmid | 7485528 |
| Wosid | WOS:A1995RZ43300015 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | PEROXYNITRITE; NITROGEN DIOXIDE; OXIDANT; SURFACE TENSION |