Exposure to mono-n-butyl phthalate disrupts the development of preimplantation embryos
Chu, DP; Tian, S; Sun, DG; Hao, CJ; Xia, HF; Ma, X
HERO ID
1639234
Reference Type
Journal Article
Year
2013
Language
English
PMID
| HERO ID | 1639234 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | Exposure to mono-n-butyl phthalate disrupts the development of preimplantation embryos |
| Authors | Chu, DP; Tian, S; Sun, DG; Hao, CJ; Xia, HF; Ma, X |
| Journal | Reproduction, Fertility and Development |
| Volume | 25 |
| Issue | 8 |
| Page Numbers | 1174-1184 |
| Abstract | Dibutyl phthalate (DBP), a widely used phthalate, is known to cause many serious diseases, especially in the reproductive system. However, little is known about the effects of its metabolite, mono-n-butyl phthalate (MBP), on preimplantation embryo development. In the present study, we found that treatment of embryos with 10⁻³ M MBP impaired developmental competency, whereas exposure to 10⁻⁴ M MBP delayed the progression of preimplantation embryos to the blastocyst stage. Furthermore, reactive oxygen species (ROS) levels in embryos were significantly increased following treatment with 10⁻³ M MBP. In addition, 10⁻³ M MBP increased apoptosis via the release of cytochrome c, whereas immunofluorescent analysis revealed that exposure of preimplantation embryos to MBP concentration-dependently (10⁻⁵, 10⁻⁴ and 10⁻³ M) decreased DNA methylation. Together, the results indicate a possible relationship between MBP exposure and developmental failure in preimplantation embryos. |
| Doi | 10.1071/RD12178 |
| Pmid | 23231764 |
| Wosid | WOS:000338923900010 |
| Url | https://search.proquest.com/docview/1663549612?accountid=171501 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | DNA methylation; reactive oxygen species |