CATALYTIC ACTIVITY OF UREASE IN NONAQUEOUS MEDIA
Puchkaev, AV; Metelitsa, DI
| HERO ID | 1631019 |
|---|---|
| In Press | No |
| Year | 1994 |
| Title | CATALYTIC ACTIVITY OF UREASE IN NONAQUEOUS MEDIA |
| Authors | Puchkaev, AV; Metelitsa, DI |
| Journal | Biochemistry (Moscow) |
| Volume | 59 |
| Issue | 1 |
| Page Numbers | 33-39 |
| Abstract | The kinetics of urease-catalyzed urea hydrolysis has been studied in mixtures of water with organic solvents (DMF, DMSO, ethanol, isopropanol, or dioxane), in detergent-free microemulsions (hexane-isopropanol-water), and inverted micelles formed by anionic, cationic, or nonionic detergents (Aerosol(R)-OT, cetyltrimethylammonium bromide, and Triton X-100, respectively), including mixed inverted micelles (Aerosol(R)-OT plus Triton X-100). The kinetic parameters of the reaction (k(cat) and K-m) and the parameter ratios were determined for all the systems of urease-catalyzed urea hydrolysis in nonaqueous media. The value of k (cat)/K-m obtained for the enzyme incorporated into the mixed micelles was maximal, suggesting that under the conditions mimicking the natural environment of urease its operation is most effective. Practical recommendations on the choice of organism solvents which ensure optimal conditions for urease-catalyzed urea hydrolysis are provided as well. |
| Wosid | WOS:A1994PK07500007 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:A1994PK07500007 |
| Is Public | Yes |
| Keyword | UREASE; AQUEOUS ORGANIC SOLVENTS; DETERGENT-FREE MICROEMULSIONS; INVERTED MICELLES; CATALYTIC ACTIVITY IN NONAQUEOUS MEDIA |