Separation of chloramphenicol intermediates by high-performance liquid chromatography on micropak-NH2 columns
Vigh, G; Inczédy, J
| HERO ID | 1616310 |
|---|---|
| In Press | No |
| Year | 1976 |
| Title | Separation of chloramphenicol intermediates by high-performance liquid chromatography on micropak-NH2 columns |
| Authors | Vigh, G; Inczédy, J |
| Journal | Journal of Chromatography A |
| Volume | 129 |
| Issue | 12/22/2020 |
| Page Numbers | 80-89 |
| Abstract | A column containing a chemically bonded phase, MicroPak-NH2, was used to seperate six components of a chloramphenicol-intermediate mixture. Two-component eluents comprising a non-hydrogen-bonding apolar solvent (cyclohexane) and a polar hydrogen-bonding solvent (dioxan, tetrahydrofuran or isopropanol) were tested. A correlation was found to exist between the values of the capacity factor (k') and the Hildebrand parameter (delta). Three-component eluents comprising cyclohexane and one of the binary mixtures dioxan-isopropanol or tetrahydrofuran-isopropanol were also studied. Optimum separation conditions with respect to analysis time, degree of resolution and detection limit were established. The system was successfully used to analyse production samples. |
| Doi | 10.1016/S0021-9673(00)87770-5 |
| Pmid | 1002810 |
| Url | https://linkinghub.elsevier.com/retrieve/pii/S0021967300877705 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |