Immunologic Tests of Specific Antibodies to Organic Acid Anhydrides
Welinder, H; Nielsen, J
HERO ID
1599573
Reference Type
Journal Article
Year
1991
Language
English
PMID
| HERO ID | 1599573 |
|---|---|
| In Press | No |
| Year | 1991 |
| Title | Immunologic Tests of Specific Antibodies to Organic Acid Anhydrides |
| Authors | Welinder, H; Nielsen, J |
| Journal | Allergy |
| Volume | 46 |
| Issue | 8 |
| Page Numbers | 601-609 |
| Abstract | Immunologic studies were conducted on sera from workers exposed to phthalic-anhydride (85449) (PA), methyltetrahydrophthalic-anhydride (26590205) (MTHPA), and hexahydrophthalic-anhydride (85427) (HHPA). The workers were exposed at four different facilities that produced alkyd resins, rocket guns, and electronic components. The effect of conjugates with different hapten densities (HD) on the outcome of skin prick tests, RAST of specific immunoglobulin-E (IgE) antibodies, and ELISA tests of specific immunoglobulin-G (IgG) antibodies were presented. The correlation between skin prick tests and RAST was examined. Human serum albumin conjugates (HSA) were prepared. Skin prick reactions to MTHPA increased with rising HD over the range of 6 to 13 moles/mole. The achieved HD was tested by spectrometric and gas chromatographic methods. In RAST of IgE antibodies MTHPA-HSA with HD six and 25 showed significantly lower bindings than conjugates with intermediate HD. A good correlation was obtained between skin prick tests and RAST. Of 234 workers tested, 45 had a skin prick reaction of over 50% of the histamine reaction. All but two of these were RAST positive. Nine RAST positive workers had no obvious skin prick reaction. However, their RAST values were low. In exposed workers, the ELISA value of specific IgG antibodies to MTHPA/HSA showed optimal values when tested with the HD 13 conjugate. The specific antibodies in workers exposed to either MTHPA or HHPA/MHHPA showed a marked cross reactivity to MTHPA/HSA, HHPA/HSA, and MHHPA/HSA as proven by skin prick tests, RAST, and RAST inhibition. In workers exposed to PA there was less cross reactivity to the other anhydrides. |
| Doi | 10.1111/j.1398-9995.1991.tb00630.x |
| Pmid | 1789402 |
| Wosid | WOS:A1991GV53400007 |
| Url | http://doi.wiley.com/10.1111/j.1398-9995.1991.tb00630.x |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | <?xml version="1.0" encoding="UTF-8"?><kw>DCN-204356</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Electronics industry</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Chemical industry workers</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Anhydrides</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Occupational exposure</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Humans</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Immune reaction</kw>; <?xml version="1.0" encoding="UTF-8"?><kw>Immunoglobulins</kw> |
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