Fabrication of non-enzymatic biosensor based on metallic catalyst-TiO2 hollow sphere nanocomposite for determining biomolecules
Kwen, HD; Yang, HS; Lee, IH; Choi, SH
| HERO ID | 1580751 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | Fabrication of non-enzymatic biosensor based on metallic catalyst-TiO2 hollow sphere nanocomposite for determining biomolecules |
| Authors | Kwen, HD; Yang, HS; Lee, IH; Choi, SH |
| Journal | Journal of Nanoscience and Nanotechnology |
| Volume | 12 |
| Issue | 7 |
| Page Numbers | 5216-5221 |
| Abstract | A PtRu@TiO2-hollow nanocomposite for the detection of biomolecules was synthesized by chemical reduction. First, poly(styrene-co-vinylphenylboronic acid), PSB, was prepared as a template (approximately 250 nm) by surfactant-free emulsion polymerization. Second, PSB/TiO2 core-shell spheres were prepared by sol-gel reaction. Finally, TiO2 hollow spheres (TiO2-H) were then formed after removing the PSB template by calcination at 450 degrees C under air atmosphere. To prepare the electrocatalyst, PtRu nanoparticles (NPs) were deposited onto the TiO2-H surface by chemical reduction. The prepared PtRu@TiO2-H nanocomposite was characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), and elemental analysis. A non-enzymatic sensor was fabricated by depositing the as-prepared PtRu@TiO2-H nanocomposite on the surface of a glassy carbon electrode (GCE), which was prepared by a hand casting method with Nafion solution as a binder. The sensor was tested as a biomolecule sensor, especially for the detection of glucose and dopamine. The cyclic voltammograms (CV) obtained during the oxidation studies revealed that the PtRu@TiO2-H nanocomposite showed better catalytic function toward the oxidation of dopamine. The sensing range of the non-enzymatic sensor for glucose was 5.0-100 mM in a phosphate buffer. The results demonstrated the potential usefulness of this bimetallic@TiO2-H bifunctional catalyst for biosensor applications. |
| Doi | 10.1166/jnn.2012.6305 |
| Pmid | 22966548 |
| Wosid | WOS:000307604700017 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Non-Enzymatic Biosensor; Biomolecular Sensing; Bimetallic Catalyst; TiO2-Hollow Nanocomposite |