ENDOCYTOSIS OF FORMALDEHYDE-DENATURED SERUM-ALBUMIN BY NONPARENCHYMAL LIVER-CELLS INVITRO
Eskild, W; Berg, T
| HERO ID | 1578985 |
|---|---|
| In Press | No |
| Year | 1984 |
| Title | ENDOCYTOSIS OF FORMALDEHYDE-DENATURED SERUM-ALBUMIN BY NONPARENCHYMAL LIVER-CELLS INVITRO |
| Authors | Eskild, W; Berg, T |
| Journal | Biochimica et Biophysica Acta |
| Volume | 803 |
| Issue | 1-2 |
| Page Numbers | 63-70 |
| Abstract | The uptake and degradation of 125I-labeled formaldehyde-denatured serum albumin in nonparenchymal rat liver cells were studied in vitro. Nonparenchymal cells bound formaldehyde-denatured serum albumin at two types of binding site, one with a high affinity and one a low affinity. The number of high affinity binding sites was approx. 10(5) per cell and the association constant, Ka 10(8) M-1. Inhibition of protein synthesis with cycloheximide did not affect the uptake and degradation of formaldehyde-denatured serum albumin suggesting reutilization of the binding sites. The presence of monensin-reduced uptake and degradation to less than 10% of control values. Pronase treatment of nonparenchymal liver cells completely abolished the uptake and degradation of the ligand. The uptake mechanism was not specific for formaldehyde-denatured serum albumin. Unlabeled acetylated, as well as malondialdehyde treated, serum albumin reduced the uptake of 125I-labeled formaldehyde-denatured serum albumin as effectively as unlabeled formaldehyde-denatured serum albumin itself. |
| Doi | 10.1016/0167-4889(84)90055-7 |
| Pmid | 6696954 |
| Wosid | WOS:A1984SH59700009 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:A1984SH59700009 |
| Is Public | Yes |
| Language Text | English |