STIMULATION BY PARAQUAT OF MICROSOMAL AND CYTOCHROME P-450-DEPENDENT OXIDATION OF GLYCEROL TO FORMALDEHYDE
Clejan, LA; Cederbaum, AI
HERO ID
1578904
Reference Type
Journal Article
Year
1993
Language
English
PMID
| HERO ID | 1578904 |
|---|---|
| In Press | No |
| Year | 1993 |
| Title | STIMULATION BY PARAQUAT OF MICROSOMAL AND CYTOCHROME P-450-DEPENDENT OXIDATION OF GLYCEROL TO FORMALDEHYDE |
| Authors | Clejan, LA; Cederbaum, AI |
| Journal | Biochemical Journal |
| Volume | 295 |
| Issue | 3 |
| Page Numbers | 781-786 |
| Abstract | BIOSIS COPYRIGHT: BIOL ABS. Glycerol can be oxidized to formaldehyde by microsomes in a reaction that is dependent on cytochrome P-450. An oxidant derived from the interaction of H2O2 with iron was responsible for oxidizing the glycerol, with P-450 suggested to be necessary to produce H2O2 and reduce non-haem iron. The effect of paraquat on formaldehyde production from glycerol and whether paraquat could replace P-450 in supporting this reaction were studied. Paraquat increased NADPH-dependent microsomal oxidation of glycerol; the stimulation was inhibited by glutathione, catalase, EDTA and desferrioxamine, but not by superoxide dismutase or hydroxyl-radical scavengers. The paraquat stimulation was also inhibited by inhibitors, substrate and ligand for P-4502E1 (pyrazole-induced P-450 isozyme), as well as by anti-(P-4502E1) IgG. These results suggest that P-450 still played an important role in glycerol oxidation, even in the presence of paraquat. Purified NADPH-cytochrome P450 reductase did not o |
| Doi | 10.1042/bj2950781 |
| Pmid | 8240292 |
| Wosid | WOS:A1993MG12100027 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:A1993MG12100027 |
| Is Public | Yes |
| Language Text | English |
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