TRANSFORMATION OF FORMALDEHYDE BY A HALOMONAS SP
Azachi, M; Henis, Y; Oren, A; Gurevich, P; Sarig, S
| HERO ID | 1578879 |
|---|---|
| In Press | No |
| Year | 1995 |
| Title | TRANSFORMATION OF FORMALDEHYDE BY A HALOMONAS SP |
| Authors | Azachi, M; Henis, Y; Oren, A; Gurevich, P; Sarig, S |
| Journal | Canadian Journal of Microbiology |
| Volume | 41 |
| Issue | 6 |
| Page Numbers | 548-553 |
| Abstract | A halotolerant Gram-negative eubacterium was isolated from soil collected at a storage site for formaldehyde near a chemical plant that uses formaldehyde in the production of glue. The strain, designated MA-C and identified as Halomonas sp. (DSM 7328), grew at salt concentrations from zero to above 20%. Halomonas sp. strain MA-C was found to be highly tolerant to formaldehyde and grew well in the presence of up to 75-100 mg/L formaldehyde. The isolate transformed formaldehyde at a high rate by means of a constitutive NAD- and glutathione-dependent formaldehyde dehydrogenase (EC 1.2.1.1). Previous exposure to formaldehyde resulted in an increase in formaldehyde dehydrogenase activity from 280-480 to 650-850 nmol . mg protein(-1) . min(-1). Formate did not accumulate in culture media enriched with formaldehyde, and formate added to cultures was rapidly metabolized. An inducible NAD-dependent formate dehydrogenase (EC 1.2.1.2) was detected (activity between 14 and 34 nmol NAD reduced mg protein(-1) . min(-1)). Radioactive labelling experiments showed that neither formaldehyde nor formate was incorporated into cell material to a significant extent. |
| Doi | 10.1139/m95-073 |
| Wosid | WOS:A1995RK58800014 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:A1995RK58800014 |
| Is Public | Yes |
| Keyword | FORMALDEHYDE; FORMATE; HALOMONAS; HALOTOLERANT |